Macrophage inhibitory cytokine MIC-1 is upregulated by short-wavelength light in cultured normal human dermal fibroblasts

Masashi Akiyama, Keiko Okano, Yoshitaka Fukada, Toshiyuki Okano

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    To better understand dermal response to visible light, we used DNA microarray analysis to search genes induced by blue or near-UV light in normal human dermal fibroblasts. Of about 12 800 transcripts analyzed, near-UV light most prominently upregulated the transcript level of Mic-1, a gene encoding a TGF-β superfamily protein. Quantitative RT-PCR and immunoblot analyses revealed that mRNA and protein levels of Mic-1 were upregulated by both short-wavelength light but not by green or red light. These results suggest that the human dermis is a site for macrophage inhibitory cytokine-1 (MIC-1) production and that visible light activates a dermal transcription cascade. Considering the role of MIC-1 in immune regulation and appetite control, photic MIC-1 regulation is of physiological importance.

    Original languageEnglish
    Pages (from-to)933-937
    Number of pages5
    JournalFEBS Letters
    Volume583
    Issue number5
    DOIs
    Publication statusPublished - 2009 Mar 4

    Fingerprint

    Growth Differentiation Factor 15
    Macrophages
    Fibroblasts
    Ultraviolet Rays
    Cytokines
    Light
    Wavelength
    Skin
    Ultraviolet radiation
    Appetite Regulation
    Methyl Green
    Microarray Analysis
    Dermis
    Oligonucleotide Array Sequence Analysis
    Genes
    Gene encoding
    Proteins
    Transcription
    Microarrays
    Polymerase Chain Reaction

    Keywords

    • Blue light
    • Dermal fibroblast
    • MIC-1
    • Microarray
    • Near-UV light

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Genetics
    • Molecular Biology
    • Structural Biology

    Cite this

    Macrophage inhibitory cytokine MIC-1 is upregulated by short-wavelength light in cultured normal human dermal fibroblasts. / Akiyama, Masashi; Okano, Keiko; Fukada, Yoshitaka; Okano, Toshiyuki.

    In: FEBS Letters, Vol. 583, No. 5, 04.03.2009, p. 933-937.

    Research output: Contribution to journalArticle

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