Abstract
To better understand dermal response to visible light, we used DNA microarray analysis to search genes induced by blue or near-UV light in normal human dermal fibroblasts. Of about 12 800 transcripts analyzed, near-UV light most prominently upregulated the transcript level of Mic-1, a gene encoding a TGF-β superfamily protein. Quantitative RT-PCR and immunoblot analyses revealed that mRNA and protein levels of Mic-1 were upregulated by both short-wavelength light but not by green or red light. These results suggest that the human dermis is a site for macrophage inhibitory cytokine-1 (MIC-1) production and that visible light activates a dermal transcription cascade. Considering the role of MIC-1 in immune regulation and appetite control, photic MIC-1 regulation is of physiological importance.
| Original language | English |
|---|---|
| Pages (from-to) | 933-937 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 583 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 Mar 4 |
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Keywords
- Blue light
- Dermal fibroblast
- MIC-1
- Microarray
- Near-UV light
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology
Cite this
Macrophage inhibitory cytokine MIC-1 is upregulated by short-wavelength light in cultured normal human dermal fibroblasts. / Akiyama, Masashi; Okano, Keiko; Fukada, Yoshitaka; Okano, Toshiyuki.
In: FEBS Letters, Vol. 583, No. 5, 04.03.2009, p. 933-937.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Macrophage inhibitory cytokine MIC-1 is upregulated by short-wavelength light in cultured normal human dermal fibroblasts
AU - Akiyama, Masashi
AU - Okano, Keiko
AU - Fukada, Yoshitaka
AU - Okano, Toshiyuki
PY - 2009/3/4
Y1 - 2009/3/4
N2 - To better understand dermal response to visible light, we used DNA microarray analysis to search genes induced by blue or near-UV light in normal human dermal fibroblasts. Of about 12 800 transcripts analyzed, near-UV light most prominently upregulated the transcript level of Mic-1, a gene encoding a TGF-β superfamily protein. Quantitative RT-PCR and immunoblot analyses revealed that mRNA and protein levels of Mic-1 were upregulated by both short-wavelength light but not by green or red light. These results suggest that the human dermis is a site for macrophage inhibitory cytokine-1 (MIC-1) production and that visible light activates a dermal transcription cascade. Considering the role of MIC-1 in immune regulation and appetite control, photic MIC-1 regulation is of physiological importance.
AB - To better understand dermal response to visible light, we used DNA microarray analysis to search genes induced by blue or near-UV light in normal human dermal fibroblasts. Of about 12 800 transcripts analyzed, near-UV light most prominently upregulated the transcript level of Mic-1, a gene encoding a TGF-β superfamily protein. Quantitative RT-PCR and immunoblot analyses revealed that mRNA and protein levels of Mic-1 were upregulated by both short-wavelength light but not by green or red light. These results suggest that the human dermis is a site for macrophage inhibitory cytokine-1 (MIC-1) production and that visible light activates a dermal transcription cascade. Considering the role of MIC-1 in immune regulation and appetite control, photic MIC-1 regulation is of physiological importance.
KW - Blue light
KW - Dermal fibroblast
KW - MIC-1
KW - Microarray
KW - Near-UV light
UR - http://www.scopus.com/inward/record.url?scp=60749120250&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60749120250&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2009.02.006
DO - 10.1016/j.febslet.2009.02.006
M3 - Article
C2 - 19302795
AN - SCOPUS:60749120250
VL - 583
SP - 933
EP - 937
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 5
ER -