Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm

Jun ichi Tanase, Takehiro Yokoo, Yuuki Matsumura, Makoto Kinoshita, Yo Kikuchi, Hirofumi Suemori, Takashi Ohyama

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Magnesium chloride and polyamines stabilize DNA and chromatin. Furthermore, they can induce nucleosome aggregation and chromatin condensation in vitro. To determine the effects of elevating the cation concentrations in the nucleus of a living cell, we microinjected various concentrations of mono-, di- and polyvalent cation solutions into the nuclei of mouse embryonic stem (ES) cells and traced their fates. Here, we show that an elevation of either MgCl2, spermidine or spermine concentration in the nucleus exerts a significant effect on mouse ES cells, and can differentiate a certain population of the cells into trophectoderm, a lineage that mouse ES cells do not normally generate, or endoderm. It is hypothesized that the cell differentiation was most probably caused by the condensation of chromatin including the Oct3/4 locus, which was induced by the elevated concentrations of these cations.

    Original languageEnglish
    Pages (from-to)764-770
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume482
    Issue number4
    DOIs
    Publication statusPublished - 2017 Jan 22

    Keywords

    • Differentiation
    • Magnesium ion
    • Mouse ES cell
    • Nuclear microinjection
    • Polyamine
    • Trophectoderm

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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