Magnetic cell separation using nano-sized bacterial magnetic particles with reconstructed magnetosome membrane

Tomoko Yoshino, Hisashi Hirabe, Masayuki Takahashi, Motoki Kuhara, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Magnetic nanoparticles produced by magnetotactic bacterium, bacterial magnetic particles (BacMPs), covered with a lipid bilayer membrane (magnetosome membrane) can be used to separate specific target cells from heterogeneous mixtures because they are easily manipulated by magnets and it is easy to display functional proteins on their surface via genetic engineering. Despite possessing unique and valuable characteristics, the potential toxicity of BacMPs to the separated cells has not been characterized in detail. Here, a novel technique was developed for the reconstruction of magnetosome membrane of BacMPs expressing protein A (protein A-BacMPs) to reduce cytotoxicity and the newly developed nanomaterial was then used for magnetic cell separation. The development of the magnetosome membrane-reconstructed protein A-BacMP was based on the characteristics of the Mms13 anchor protein, which strongly binds to the magnetite surface of BacMPs. Treatment of protein A-BacMPs with detergents removed contaminating proteins but did not affect retention of Mms13-protein A fusion proteins. The particle surfaces were then reconstructed with phosphatidylcholine. The protein A-BacMPs containing reconstructed magnetosome membranes remained dispersible and retained the ability to immobilize antibody. In addition, they contained few membrane surface proteins and endotoxins, which were observed on non-treated protein A-BacMPs. Magnetic separation of monocytes and B-rymphocytes from the peripheral blood was achieved with high purity using magnetosome membrane-reconstructed protein A-BacMPs.

Original languageEnglish
Pages (from-to)470-477
Number of pages8
JournalBiotechnology and Bioengineering
Volume101
Issue number3
DOIs
Publication statusPublished - 2008 Oct 15
Externally publishedYes

Fingerprint

Magnetosomes
Cell Separation
Staphylococcal Protein A
Proteins
Membranes
Membrane Proteins
Ferrosoferric Oxide
Genetic engineering
Magnetic separation
Lipid bilayers
Genetic Engineering
Magnets
Nanostructures
Lipid Bilayers
Cytotoxicity
Membrane Lipids
Anchors
Phosphatidylcholines
Nanostructured materials
Endotoxins

Keywords

  • Cell separation
  • Magnetic nanoparticle
  • Membrane
  • Phospholipid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Magnetic cell separation using nano-sized bacterial magnetic particles with reconstructed magnetosome membrane. / Yoshino, Tomoko; Hirabe, Hisashi; Takahashi, Masayuki; Kuhara, Motoki; Takeyama, Haruko; Matsunaga, Tadashi.

In: Biotechnology and Bioengineering, Vol. 101, No. 3, 15.10.2008, p. 470-477.

Research output: Contribution to journalArticle

Yoshino, Tomoko ; Hirabe, Hisashi ; Takahashi, Masayuki ; Kuhara, Motoki ; Takeyama, Haruko ; Matsunaga, Tadashi. / Magnetic cell separation using nano-sized bacterial magnetic particles with reconstructed magnetosome membrane. In: Biotechnology and Bioengineering. 2008 ; Vol. 101, No. 3. pp. 470-477.
@article{64e129d544d4464285742a7da4a2873b,
title = "Magnetic cell separation using nano-sized bacterial magnetic particles with reconstructed magnetosome membrane",
abstract = "Magnetic nanoparticles produced by magnetotactic bacterium, bacterial magnetic particles (BacMPs), covered with a lipid bilayer membrane (magnetosome membrane) can be used to separate specific target cells from heterogeneous mixtures because they are easily manipulated by magnets and it is easy to display functional proteins on their surface via genetic engineering. Despite possessing unique and valuable characteristics, the potential toxicity of BacMPs to the separated cells has not been characterized in detail. Here, a novel technique was developed for the reconstruction of magnetosome membrane of BacMPs expressing protein A (protein A-BacMPs) to reduce cytotoxicity and the newly developed nanomaterial was then used for magnetic cell separation. The development of the magnetosome membrane-reconstructed protein A-BacMP was based on the characteristics of the Mms13 anchor protein, which strongly binds to the magnetite surface of BacMPs. Treatment of protein A-BacMPs with detergents removed contaminating proteins but did not affect retention of Mms13-protein A fusion proteins. The particle surfaces were then reconstructed with phosphatidylcholine. The protein A-BacMPs containing reconstructed magnetosome membranes remained dispersible and retained the ability to immobilize antibody. In addition, they contained few membrane surface proteins and endotoxins, which were observed on non-treated protein A-BacMPs. Magnetic separation of monocytes and B-rymphocytes from the peripheral blood was achieved with high purity using magnetosome membrane-reconstructed protein A-BacMPs.",
keywords = "Cell separation, Magnetic nanoparticle, Membrane, Phospholipid",
author = "Tomoko Yoshino and Hisashi Hirabe and Masayuki Takahashi and Motoki Kuhara and Haruko Takeyama and Tadashi Matsunaga",
year = "2008",
month = "10",
day = "15",
doi = "10.1002/bit.21912",
language = "English",
volume = "101",
pages = "470--477",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "3",

}

TY - JOUR

T1 - Magnetic cell separation using nano-sized bacterial magnetic particles with reconstructed magnetosome membrane

AU - Yoshino, Tomoko

AU - Hirabe, Hisashi

AU - Takahashi, Masayuki

AU - Kuhara, Motoki

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

PY - 2008/10/15

Y1 - 2008/10/15

N2 - Magnetic nanoparticles produced by magnetotactic bacterium, bacterial magnetic particles (BacMPs), covered with a lipid bilayer membrane (magnetosome membrane) can be used to separate specific target cells from heterogeneous mixtures because they are easily manipulated by magnets and it is easy to display functional proteins on their surface via genetic engineering. Despite possessing unique and valuable characteristics, the potential toxicity of BacMPs to the separated cells has not been characterized in detail. Here, a novel technique was developed for the reconstruction of magnetosome membrane of BacMPs expressing protein A (protein A-BacMPs) to reduce cytotoxicity and the newly developed nanomaterial was then used for magnetic cell separation. The development of the magnetosome membrane-reconstructed protein A-BacMP was based on the characteristics of the Mms13 anchor protein, which strongly binds to the magnetite surface of BacMPs. Treatment of protein A-BacMPs with detergents removed contaminating proteins but did not affect retention of Mms13-protein A fusion proteins. The particle surfaces were then reconstructed with phosphatidylcholine. The protein A-BacMPs containing reconstructed magnetosome membranes remained dispersible and retained the ability to immobilize antibody. In addition, they contained few membrane surface proteins and endotoxins, which were observed on non-treated protein A-BacMPs. Magnetic separation of monocytes and B-rymphocytes from the peripheral blood was achieved with high purity using magnetosome membrane-reconstructed protein A-BacMPs.

AB - Magnetic nanoparticles produced by magnetotactic bacterium, bacterial magnetic particles (BacMPs), covered with a lipid bilayer membrane (magnetosome membrane) can be used to separate specific target cells from heterogeneous mixtures because they are easily manipulated by magnets and it is easy to display functional proteins on their surface via genetic engineering. Despite possessing unique and valuable characteristics, the potential toxicity of BacMPs to the separated cells has not been characterized in detail. Here, a novel technique was developed for the reconstruction of magnetosome membrane of BacMPs expressing protein A (protein A-BacMPs) to reduce cytotoxicity and the newly developed nanomaterial was then used for magnetic cell separation. The development of the magnetosome membrane-reconstructed protein A-BacMP was based on the characteristics of the Mms13 anchor protein, which strongly binds to the magnetite surface of BacMPs. Treatment of protein A-BacMPs with detergents removed contaminating proteins but did not affect retention of Mms13-protein A fusion proteins. The particle surfaces were then reconstructed with phosphatidylcholine. The protein A-BacMPs containing reconstructed magnetosome membranes remained dispersible and retained the ability to immobilize antibody. In addition, they contained few membrane surface proteins and endotoxins, which were observed on non-treated protein A-BacMPs. Magnetic separation of monocytes and B-rymphocytes from the peripheral blood was achieved with high purity using magnetosome membrane-reconstructed protein A-BacMPs.

KW - Cell separation

KW - Magnetic nanoparticle

KW - Membrane

KW - Phospholipid

UR - http://www.scopus.com/inward/record.url?scp=53549135213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53549135213&partnerID=8YFLogxK

U2 - 10.1002/bit.21912

DO - 10.1002/bit.21912

M3 - Article

C2 - 18421798

AN - SCOPUS:53549135213

VL - 101

SP - 470

EP - 477

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 3

ER -