Magnetic control of bacterial magnetite-myosin conjugate movement on actin cables

T. Tanaka, H. Yamasaki, N. Tsujimura, N. Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The control of actin-myosin movement using a bacterial magnetic particle (BMP) which was synthesized intracellularly by magnetic bacteria was investigated. Myosin immobilized on the BMP (myosin-BMP conjugate) was prepared with heterobifunctional reagents, Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3′-(2-pyridyldithio)-propionamido)hexanoate) and Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate). When a magnetic force was applied in the same direction as the myosin-BMP conjugate movement (negative magnetic force) at 22-23°C, the velocity increased from 2.0 μm s-1 to 7.5 μm s-1. When a magnetic force was applied in the opposite direction to the myosin-BMP conjugate movement (positive magnetic force), the velocity decreased from 10.7 μm s-1 to 0 μm s-1. These results indicate that the sliding force of the myosin-BMP conjugate caused by the actin-myosin interaction can be controlled using a neodymium-boron (Nd-B) magnet. It will be possible to develop a nano-sized actuator that can be controlled by the external magnetic force. This system could also be applicable to analysis of the dynamics of a single myosin molecule.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalMaterials Science and Engineering C
Volume5
Issue number2 C
Publication statusPublished - 1997 Dec
Externally publishedYes

Fingerprint

magnetic control
Ferrosoferric Oxide
myosins
Magnetite
Myosins
magnetite
cables
Actins
Cables
Neodymium
Cyclohexane
Magnets
Boron
Bacteria
Actuators
Molecules
neodymium
cyclohexane
bacteria
carboxylates

Keywords

  • Actin cables
  • Bacterial magnetic particle
  • Chara corallina
  • Myosin

ASJC Scopus subject areas

  • Biomaterials

Cite this

Magnetic control of bacterial magnetite-myosin conjugate movement on actin cables. / Tanaka, T.; Yamasaki, H.; Tsujimura, N.; Nakamura, N.; Matsunaga, Tadashi.

In: Materials Science and Engineering C, Vol. 5, No. 2 C, 12.1997, p. 121-124.

Research output: Contribution to journalArticle

Tanaka, T, Yamasaki, H, Tsujimura, N, Nakamura, N & Matsunaga, T 1997, 'Magnetic control of bacterial magnetite-myosin conjugate movement on actin cables', Materials Science and Engineering C, vol. 5, no. 2 C, pp. 121-124.
Tanaka, T. ; Yamasaki, H. ; Tsujimura, N. ; Nakamura, N. ; Matsunaga, Tadashi. / Magnetic control of bacterial magnetite-myosin conjugate movement on actin cables. In: Materials Science and Engineering C. 1997 ; Vol. 5, No. 2 C. pp. 121-124.
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