Magnetic separation of CD14+ cells using antibody binding with protein A expressed on bacterial magnetic particles for generating dendritic cells

Tadashi Matsunaga, Masayuki Takahashi, Tomoko Yoshino, Motoki Kuhara, Haruko Takeyama

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Herein the potential of a highly efficient cell separation system using bacterial magnetic particles expressing protein A (protein A-BacMPs) was demonstrated. Protein A was expressed on BacMPs using the transmembrane proteins Mms13 and MagA as anchor molecules. The evaluations of the numbers of bound antibody molecules and binding capabilities of the protein A-BacMPs using Mms13 indicated that the antibodies were efficiently introduced into protein A-BacMPs using Mms13 in comparison to MagA. In addition, the recovery ratio of the target cells on the magnetic cell separation system was enhanced by using protein A-BacMPs with Mms13. Using positive selection against peripheral blood mononuclear cells, the CD14+ cells were separated at a purity of more than 99% by protein A-BacMPs using Mms13. Furthermore, in the evaluation of the influence of protein A-BacMPs on the separated cells, the CD14+ cells separated using protein A-BacMPs and were successfully differentiated into dendritic cells.

Original languageEnglish
Pages (from-to)1019-1025
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number4
DOIs
Publication statusPublished - 2006 Dec 1

Keywords

  • Antibody
  • Bacterial magnetic particle
  • CD14 cell
  • Dendritic cell
  • Magnetic cell separation
  • Protein A

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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