Abstract
Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.
| Original language | English |
|---|---|
| Pages (from-to) | 23-30 |
| Number of pages | 8 |
| Journal | Cell Structure and Function |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
| Externally published | Yes |
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Keywords
- Bacterial magnetic particles (BacMPs)
- Cell separation
- Hematopoietic progenitor cells
- Podocalyxin-like protein 1 (PCLP1)
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Physiology
Cite this
Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles. / Kuhara, Motoki; Yoshino, Tomoko; Shiokawa, Miho; Okabe, Tomoya; Mizoguchi, Shinji; Yabuhara, Akihiko; Takeyama, Haruko; Matsunaga, Tadashi.
In: Cell Structure and Function, Vol. 34, No. 1, 2009, p. 23-30.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles
AU - Kuhara, Motoki
AU - Yoshino, Tomoko
AU - Shiokawa, Miho
AU - Okabe, Tomoya
AU - Mizoguchi, Shinji
AU - Yabuhara, Akihiko
AU - Takeyama, Haruko
AU - Matsunaga, Tadashi
PY - 2009
Y1 - 2009
N2 - Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.
AB - Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.
KW - Bacterial magnetic particles (BacMPs)
KW - Cell separation
KW - Hematopoietic progenitor cells
KW - Podocalyxin-like protein 1 (PCLP1)
UR - http://www.scopus.com/inward/record.url?scp=66149113321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66149113321&partnerID=8YFLogxK
U2 - 10.1247/csf.08043
DO - 10.1247/csf.08043
M3 - Article
VL - 34
SP - 23
EP - 30
JO - Cell Structure and Function
JF - Cell Structure and Function
SN - 0386-7196
IS - 1
ER -