Abstract
Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.
Original language | English |
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Pages (from-to) | 23-30 |
Number of pages | 8 |
Journal | Cell Structure and Function |
Volume | 34 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2009 |
Externally published | Yes |
Keywords
- Bacterial magnetic particles (BacMPs)
- Cell separation
- Hematopoietic progenitor cells
- Podocalyxin-like protein 1 (PCLP1)
ASJC Scopus subject areas
- Physiology
- Molecular Biology
- Cell Biology