Abstract
A helically shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The growth medium contained succinate, nitrate and ferric malate as the carbon, nitrogen and iron sources, respectively. The magnetic bacterium, designated AMB-1, was able to grow in free gaseous exchange with an air atmosphere. When cells were grown aerobically on agar, oxidase activity was present, and white non-magnetic colonies, which did not show catalase activity, were formed. The stationary phase of growth was reached 4-5 days later at a cell concentration of 1.4×109 cells/ml in liquid culture when an initial cell concentration of 105 cells/ml was employed. After ultrasonic disruption of harvested cells, 2.6 mg bacterial magnetite was obtained from a 11 culture.
| Original language | English |
|---|---|
| Pages (from-to) | 651-655 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 35 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1991 Aug |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
- Bioengineering
- Biotechnology
Cite this
Magnetite formation by a magnetic bacterium capable of growing aerobically. / Matsunaga, Tadashi; Sakaguchi, Toshifumi; Tadakoro, Fumihiko.
In: Applied Microbiology and Biotechnology, Vol. 35, No. 5, 08.1991, p. 651-655.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Magnetite formation by a magnetic bacterium capable of growing aerobically
AU - Matsunaga, Tadashi
AU - Sakaguchi, Toshifumi
AU - Tadakoro, Fumihiko
PY - 1991/8
Y1 - 1991/8
N2 - A helically shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The growth medium contained succinate, nitrate and ferric malate as the carbon, nitrogen and iron sources, respectively. The magnetic bacterium, designated AMB-1, was able to grow in free gaseous exchange with an air atmosphere. When cells were grown aerobically on agar, oxidase activity was present, and white non-magnetic colonies, which did not show catalase activity, were formed. The stationary phase of growth was reached 4-5 days later at a cell concentration of 1.4×109 cells/ml in liquid culture when an initial cell concentration of 105 cells/ml was employed. After ultrasonic disruption of harvested cells, 2.6 mg bacterial magnetite was obtained from a 11 culture.
AB - A helically shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The growth medium contained succinate, nitrate and ferric malate as the carbon, nitrogen and iron sources, respectively. The magnetic bacterium, designated AMB-1, was able to grow in free gaseous exchange with an air atmosphere. When cells were grown aerobically on agar, oxidase activity was present, and white non-magnetic colonies, which did not show catalase activity, were formed. The stationary phase of growth was reached 4-5 days later at a cell concentration of 1.4×109 cells/ml in liquid culture when an initial cell concentration of 105 cells/ml was employed. After ultrasonic disruption of harvested cells, 2.6 mg bacterial magnetite was obtained from a 11 culture.
UR - http://www.scopus.com/inward/record.url?scp=0025898964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025898964&partnerID=8YFLogxK
U2 - 10.1007/BF00169632
DO - 10.1007/BF00169632
M3 - Article
AN - SCOPUS:0025898964
VL - 35
SP - 651
EP - 655
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -