Maintenance of broad host range vector pKT230 in marine unicellular cyanobacteria

Koji Sode, Masahiro Tatara, Shigeo Ogawa, Tadashi Matsunaga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The host range of pKT230 in marine unicellular cyanobacteria was investigated. The broad host range vector, pKT230, was successfully transferred by transconjugation to Synechococcus sp. PCC7335, NKBG15031a and Synechocystis 7a. pKT230 was found to be maintained as an autonomous replicon in strains NKBG15031a and 7a. pKT230 stability in Synechococcus sp. NKBG15041c, in which pKT230 was found to be maintained as an autonomous replicon in our previous report [1], was also studied. In the absence of selection pressure, after 120 h of cultivation, about 50% of cells lost the plasmid. In the presence of selection pressure (kanamycin 25 μg ml-), after 120 h of cultivation, more than 90% of the cells harboured the plasmid.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalFEMS Microbiology Letters
Volume99
Issue number1
Publication statusPublished - 1992 Nov 15
Externally publishedYes

Keywords

  • Broad host range vector
  • Marine cyanobacteria
  • Plasmid stability
  • Transconjugation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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  • Cite this

    Sode, K., Tatara, M., Ogawa, S., & Matsunaga, T. (1992). Maintenance of broad host range vector pKT230 in marine unicellular cyanobacteria. FEMS Microbiology Letters, 99(1), 73-78.