Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment

Kazuhide Asakawa, Mika Toya, Masamitsu Sato, Muneyoshi Kanai, Kazunori Kume, Tetsuya Goshima, Miguel Angel Garcia, Dai Hirata, Takashi Toda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bipolar microtubule attachment is central to genome stability. Here, we investigate the mitotic role of the fission yeast EB1 homologue Mal3. Mal3 shows dynamic inward movement along the spindle, initial emergence at the spindle pole body (SPB) and translocation towards the equatorial plane, followed by sudden disappearance. Deletion of Mal3 results in early mitotic delay, which is dependent on the Bub1, but not the Mad2, spindle checkpoint. Consistently, Bub1, but not Mad2, shows prolonged kinetochore localization. Double mutants between mal3 and a subset of checkpoint mutants, including bub1, bub3, mad3 and mph1, but not mad1 or mad2, show massive chromosome mis-segregation defects. In mal3bub1 mutants, both sister centromeres tend to remain in close proximity to one of the separating SPBs. Further analysis indicates that mis-segregated centromeres are exclusively associated with the mother SPB. Mal3, therefore, has a role in preventing monopolar attachment in cooperation with the Bub1/Bub3/Mad3/Mph1-dependent checkpoint.

Original languageEnglish
Pages (from-to)1194-1200
Number of pages7
JournalEMBO Reports
Volume6
Issue number12
DOIs
Publication statusPublished - 2005 Dec
Externally publishedYes

Fingerprint

Spindle Pole Bodies
Schizosaccharomyces
Centromere
Yeast
Poles
Kinetochores
Chromosome Segregation
Genomic Instability
Chromosomes
Microtubules
Genes
Defects

Keywords

  • Bipolar attachment
  • EB1
  • Fission yeast
  • Kinetochore
  • Spindle checkpoint

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment. / Asakawa, Kazuhide; Toya, Mika; Sato, Masamitsu; Kanai, Muneyoshi; Kume, Kazunori; Goshima, Tetsuya; Garcia, Miguel Angel; Hirata, Dai; Toda, Takashi.

In: EMBO Reports, Vol. 6, No. 12, 12.2005, p. 1194-1200.

Research output: Contribution to journalArticle

Asakawa, Kazuhide ; Toya, Mika ; Sato, Masamitsu ; Kanai, Muneyoshi ; Kume, Kazunori ; Goshima, Tetsuya ; Garcia, Miguel Angel ; Hirata, Dai ; Toda, Takashi. / Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment. In: EMBO Reports. 2005 ; Vol. 6, No. 12. pp. 1194-1200.
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