Mammalian mitochondrial methionyl-tRNA transformylase from bovine liver. Purification, characterization, and gene structure

Nono Takeuchi, Makoto Kawakami, Akira Omori, Takuya Ueda, Linda L. Spremulli, Kimitsuna Watanabe

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The mammalian mitochondrial methionyl-tRNA transformylase (MTF(mt)) was partially purified 2,200-fold from bovine liver mitochondria using column chromatography. The polypeptide responsible for MTF(mt) activity was excised from a sodium dodecyl sulfate-polyacrylamide gel and the amino acid sequences of several peptides were determined. The cDNA encoding bovine MTF(mt) was obtained and its nucleotide sequence was determined. The deduced amino acid sequence of the mature form of MTF(mt) consists of 357 amino acid residues. This sequence is about 30% identical to the corresponding Escherichia coli and yeast mitochondrial MTFs. Kinetic parameters governing the formylation of various tRNAs were obtained. Bovine MTF(mt) formylates its homologous mitochondrial methionyl-tRNA and the E. coli initiator methionyl-tRNA (Met- tRNA(fMet)) with essentially equal efficiency. The E. coli elongator methionyl-tRNA (Met-tRNA(mMet)) was also formylated although with somewhat less favorable kinetics. These results suggest that the substrate specificity of MTF(mt) is not as rigid as that of the E. coli MTF which clearly discriminates between the bacterial initiator and elongator Met-tRNAs. These observations are discussed in terms of the presence of a single tRNA(Met) gene in mammalian mitochondria.

Original languageEnglish
Pages (from-to)15085-15090
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number24
DOIs
Publication statusPublished - 1998 Jun 12
Externally publishedYes

Fingerprint

methionyl-tRNA formyltransferase
RNA, Transfer, Met
Liver
Escherichia coli
Purification
Transfer RNA
Genes
Mitochondria
Amino Acids
Amino Acid Sequence
Peptides
Column chromatography
Kinetic parameters
Sodium Dodecyl Sulfate
Yeast
Liver Mitochondrion
Substrate Specificity
Nucleotides
Complementary DNA
Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mammalian mitochondrial methionyl-tRNA transformylase from bovine liver. Purification, characterization, and gene structure. / Takeuchi, Nono; Kawakami, Makoto; Omori, Akira; Ueda, Takuya; Spremulli, Linda L.; Watanabe, Kimitsuna.

In: Journal of Biological Chemistry, Vol. 273, No. 24, 12.06.1998, p. 15085-15090.

Research output: Contribution to journalArticle

Takeuchi, Nono ; Kawakami, Makoto ; Omori, Akira ; Ueda, Takuya ; Spremulli, Linda L. ; Watanabe, Kimitsuna. / Mammalian mitochondrial methionyl-tRNA transformylase from bovine liver. Purification, characterization, and gene structure. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 24. pp. 15085-15090.
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