TY - JOUR
T1 - Mangromicins A and B
T2 - Structure and antitrypanosomal activity of two new cyclopentadecane compounds from Lechevalieria aerocolonigenes K10-0216
AU - Nakashima, Takuji
AU - Iwatsuki, Masato
AU - Ochiai, Junya
AU - Kamiya, Yoshiyuki
AU - Nagai, Kenichiro
AU - Matsumoto, Atsuko
AU - Ishiyama, Aki
AU - Otoguro, Kazuhiko
AU - Shiomi, Kazuro
AU - Takahashi, Yoko
AU - Omura, Satoshi
N1 - Funding Information:
This study was supported, in part, by funds from Quality Assurance Framework of Higher Education from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and the institute for Fermentation (IFO), Osaka, Japan. We are grateful to Ms Noriko Sato, School of Pharmacy, Kitasato University, for measurements of NMR spectra, Ms Miyuki Namatame and Dr Tomoyasu Hirose, Kitasato Institute for Life Sciences, Kitasato University, for antitrypanosomal and cytotoxic assay and advice on the structure, respectively.
PY - 2014/3
Y1 - 2014/3
N2 - Two new cyclopentadecane antibiotics, named mangromicins A and B, were separated out from the culture broth of Lechevalieria aerocolonigenes K10-0216 by Diaion HP-20, silica gel and ODS column chromatography, and were finally purified by HPLC. The chemical structures of the two novel compounds were elucidated by instrumental analyses, including various NMR, MS and X-ray crystallography. Mangromicins A and B consist of cyclopentadecane skeletons with a tetrahydrofuran unit and a 5,6-dihydro-4-hydroxy-2-pyrone moiety. Mangromicins A and B showed in vitro antitrypanosomal activity with IC 50 values of 2.4 and 43.4 μg ml -1, respectively. The IC 50 values of both compounds were lower than those of cytotoxicity against MRC-5 human fetal lung fibroblast cells.
AB - Two new cyclopentadecane antibiotics, named mangromicins A and B, were separated out from the culture broth of Lechevalieria aerocolonigenes K10-0216 by Diaion HP-20, silica gel and ODS column chromatography, and were finally purified by HPLC. The chemical structures of the two novel compounds were elucidated by instrumental analyses, including various NMR, MS and X-ray crystallography. Mangromicins A and B consist of cyclopentadecane skeletons with a tetrahydrofuran unit and a 5,6-dihydro-4-hydroxy-2-pyrone moiety. Mangromicins A and B showed in vitro antitrypanosomal activity with IC 50 values of 2.4 and 43.4 μg ml -1, respectively. The IC 50 values of both compounds were lower than those of cytotoxicity against MRC-5 human fetal lung fibroblast cells.
KW - Antitrypanosomal activity
KW - Lechevalieria aerocolonigenes
KW - Mangromicins
KW - Mangrove sediment
KW - Physicochemical screening
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U2 - 10.1038/ja.2013.129
DO - 10.1038/ja.2013.129
M3 - Article
C2 - 24326338
AN - SCOPUS:84897130454
SN - 0021-8820
VL - 67
SP - 253
EP - 260
JO - Journal of Antibiotics
JF - Journal of Antibiotics
IS - 3
ER -