Abstract
Reactive oxygen and nitrogen species generated either as products of aerobic metabolism or as a consequence of environmental mutagens, oxidatively modify DNA. Formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (endo III) or their functional mammalian homologues repair 7,8-dihydro-8-oxoguanine (8-oxoG) and damaged pyrimidines, respectively, to curb the deleterious effects of oxidative DNA alterations. A single bout of physical exercise can induce oxidative DNA damage. However, its effect on the activity of repair enzymes is not known. Here we report that the activity of a functional homolog of Fpg, human 8-oxoG DNA glycosylase (hOGG1), is increased significantly, as measured by the excision of 32P labeled damaged oligonucleotide, in human skeletal muscle after a marathon race. The AP site repair enzyme did not change significantly. Despite the large individual differences among the six subjects measured, data suggest that a single-bout of aerobic exercise increases the activity of hOGG1 which is responsible for the excision of 8-oxoG. The up-regulation of DNA repair enzymes might be an important part of the regular exercise induced adaptation process.
| Original language | English |
|---|---|
| Pages (from-to) | 1627-1633 |
| Number of pages | 7 |
| Journal | Life Sciences |
| Volume | 72 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2003 Feb 21 |
| Externally published | Yes |
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Keywords
- Adaptation
- Base excision repair
- DNA damage
- DNA repair
- Exercise
- Oxidative stress
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Marathon running alters the DNA base excision repair in human skeletal muscle. / Radak, Zsolt; Apor, Peter; Pucsok, Jozsef; Berkes, Istvan; Ogonovszky, Helga; Pavlik, Gabor; Nakamoto, Hideko; Goto, Sataro.
In: Life Sciences, Vol. 72, No. 14, 21.02.2003, p. 1627-1633.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Marathon running alters the DNA base excision repair in human skeletal muscle
AU - Radak, Zsolt
AU - Apor, Peter
AU - Pucsok, Jozsef
AU - Berkes, Istvan
AU - Ogonovszky, Helga
AU - Pavlik, Gabor
AU - Nakamoto, Hideko
AU - Goto, Sataro
PY - 2003/2/21
Y1 - 2003/2/21
N2 - Reactive oxygen and nitrogen species generated either as products of aerobic metabolism or as a consequence of environmental mutagens, oxidatively modify DNA. Formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (endo III) or their functional mammalian homologues repair 7,8-dihydro-8-oxoguanine (8-oxoG) and damaged pyrimidines, respectively, to curb the deleterious effects of oxidative DNA alterations. A single bout of physical exercise can induce oxidative DNA damage. However, its effect on the activity of repair enzymes is not known. Here we report that the activity of a functional homolog of Fpg, human 8-oxoG DNA glycosylase (hOGG1), is increased significantly, as measured by the excision of 32P labeled damaged oligonucleotide, in human skeletal muscle after a marathon race. The AP site repair enzyme did not change significantly. Despite the large individual differences among the six subjects measured, data suggest that a single-bout of aerobic exercise increases the activity of hOGG1 which is responsible for the excision of 8-oxoG. The up-regulation of DNA repair enzymes might be an important part of the regular exercise induced adaptation process.
AB - Reactive oxygen and nitrogen species generated either as products of aerobic metabolism or as a consequence of environmental mutagens, oxidatively modify DNA. Formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (endo III) or their functional mammalian homologues repair 7,8-dihydro-8-oxoguanine (8-oxoG) and damaged pyrimidines, respectively, to curb the deleterious effects of oxidative DNA alterations. A single bout of physical exercise can induce oxidative DNA damage. However, its effect on the activity of repair enzymes is not known. Here we report that the activity of a functional homolog of Fpg, human 8-oxoG DNA glycosylase (hOGG1), is increased significantly, as measured by the excision of 32P labeled damaged oligonucleotide, in human skeletal muscle after a marathon race. The AP site repair enzyme did not change significantly. Despite the large individual differences among the six subjects measured, data suggest that a single-bout of aerobic exercise increases the activity of hOGG1 which is responsible for the excision of 8-oxoG. The up-regulation of DNA repair enzymes might be an important part of the regular exercise induced adaptation process.
KW - Adaptation
KW - Base excision repair
KW - DNA damage
KW - DNA repair
KW - Exercise
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=0037458406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037458406&partnerID=8YFLogxK
U2 - 10.1016/S0024-3205(02)02476-1
DO - 10.1016/S0024-3205(02)02476-1
M3 - Article
C2 - 12551751
AN - SCOPUS:0037458406
VL - 72
SP - 1627
EP - 1633
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 14
ER -