Marine diatom, Navicula sp. strain JPCC DA0580 and marine green alga, Chlorella sp. Strain NKG400014 as potential sources for biodiesel production

Mitsufumi Matsumoto, Hiroshi Sugiyama, Yoshiaki Maeda, Reiko Sato, Tsuyoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Marine diatom, strain JPCC DA0580, and marine green microalga strain NKG400014 were selected as high neutral lipid-producers from marine microalgal culture collection toward biodiesel production. These strains were tentatively identified as Navicula sp. and Chlorella sp., respectively, by 18S rDNA analysis. Growth and lipid accumulation conditions of both strains were analyzed by changing nutrient concentrations in growth media and initial illuminance intensity. The highest productivity of fatty acid methyl ester (FAME) reached to 154 mg/L/week for NKG400014 and 185 mg/L/week for JPCC DA0580. Gas chromatography/mass spectrometry analysis indicates that FAME fraction from NKG400014 mainly contained 9-12-15-octadecatrienoate (C18:3) and that from JPCC DA0580 mainly contained methyl palmitate (C16:0) and methyl palmitoleate (C16:1). Furthermore, calorimetric analysis revealed that the energy content of strain was 4,233±55 kcal/kg (i.e., 15.9±0.2 MJ/kg) for NKG400014 and 6,423±139 kcal/mg (i.e., 26.9±0.6 MJ/kg) for JPCC DA0580, respectively. The value from JPCC DA0580 was equivalent to that of coal. The strains NKG400014 and JPCC DA0580 will become a promising resource that can grow as dominant species in the open ocean toward production of both liquid and solid biofuels.

Original languageEnglish
Pages (from-to)483-490
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume161
Issue number1-8
DOIs
Publication statusPublished - 2010 May
Externally publishedYes

Fingerprint

Chlorella
Diatoms
Chlorophyta
Biofuels
Algae
Biodiesel
Esters
Fatty Acids
Lipids
Coal
Growth
Ribosomal DNA
Oceans and Seas
Gas Chromatography-Mass Spectrometry
Fatty acids
Food
Gas chromatography
Nutrients
Mass spectrometry
Productivity

Keywords

  • Biodiesel
  • Calorimetry
  • Fatty acid methyl ester
  • Microalgae
  • Triglyceride

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Marine diatom, Navicula sp. strain JPCC DA0580 and marine green alga, Chlorella sp. Strain NKG400014 as potential sources for biodiesel production. / Matsumoto, Mitsufumi; Sugiyama, Hiroshi; Maeda, Yoshiaki; Sato, Reiko; Tanaka, Tsuyoshi; Matsunaga, Tadashi.

In: Applied Biochemistry and Biotechnology, Vol. 161, No. 1-8, 05.2010, p. 483-490.

Research output: Contribution to journalArticle

Matsumoto, Mitsufumi ; Sugiyama, Hiroshi ; Maeda, Yoshiaki ; Sato, Reiko ; Tanaka, Tsuyoshi ; Matsunaga, Tadashi. / Marine diatom, Navicula sp. strain JPCC DA0580 and marine green alga, Chlorella sp. Strain NKG400014 as potential sources for biodiesel production. In: Applied Biochemistry and Biotechnology. 2010 ; Vol. 161, No. 1-8. pp. 483-490.
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AU - Sugiyama, Hiroshi

AU - Maeda, Yoshiaki

AU - Sato, Reiko

AU - Tanaka, Tsuyoshi

AU - Matsunaga, Tadashi

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