Mass Culture of Magnetic Bacteria and Their Application to Flow Type Immunoassays

Tadashi Matsunaga, F. Tadokoro, N. Nakamura

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Isolated helical shaped magnetotactic bacteria were cultured in a medium containing succinate, nitrate, and ferric malate as carbon, nitrogen, and iron sources, respectively. The magnetotactic bacteria could grow aerobically. The cells which grew aerobically had oxidase activity. An initial inoculum of 105 cells/ml was used Stationary phase was reached (1.4×109 cells/ml) after 4–5 days growth. When the cells were disrupted using ultrasonication, 2.6 mg bacterial magnetites were obtained from 1 I culture of magnetotactic bacteria. The detection of mouse IgG was carried out using FITC conjugated anti-mouse IgG immobilized on bacterial magnetites and a flow injection system with a fluorescence spectrophotometer. Relative fluorescence intensity correlated linearly with the concentration of mouse IgG in the range from 0.5-100 ng/ml and the measurements were established within 2 min using this system.

Original languageEnglish
Pages (from-to)1557-1559
Number of pages3
JournalIEEE Transactions on Magnetics
Volume26
Issue number5
DOIs
Publication statusPublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Physics and Astronomy (miscellaneous)

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