Abstract
Isolated helical shaped magnetotactic bacteria were cultured in a medium containing succinate, nitrate, and ferric malate as carbon, nitrogen, and iron sources, respectively. The magnetotactic bacteria could grow aerobically. The cells which grew aerobically had oxidase activity. An initial inoculum of 105 cells/ml was used Stationary phase was reached (1.4×109 cells/ml) after 4–5 days growth. When the cells were disrupted using ultrasonication, 2.6 mg bacterial magnetites were obtained from 1 I culture of magnetotactic bacteria. The detection of mouse IgG was carried out using FITC conjugated anti-mouse IgG immobilized on bacterial magnetites and a flow injection system with a fluorescence spectrophotometer. Relative fluorescence intensity correlated linearly with the concentration of mouse IgG in the range from 0.5-100 ng/ml and the measurements were established within 2 min using this system.
| Original language | English |
|---|---|
| Pages (from-to) | 1557-1559 |
| Number of pages | 3 |
| Journal | IEEE Transactions on Magnetics |
| Volume | 26 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy (miscellaneous)
Cite this
Mass Culture of Magnetic Bacteria and Their Application to Flow Type Immunoassays. / Matsunaga, Tadashi; Tadokoro, F.; Nakamura, N.
In: IEEE Transactions on Magnetics, Vol. 26, No. 5, 1990, p. 1557-1559.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mass Culture of Magnetic Bacteria and Their Application to Flow Type Immunoassays
AU - Matsunaga, Tadashi
AU - Tadokoro, F.
AU - Nakamura, N.
PY - 1990
Y1 - 1990
N2 - Isolated helical shaped magnetotactic bacteria were cultured in a medium containing succinate, nitrate, and ferric malate as carbon, nitrogen, and iron sources, respectively. The magnetotactic bacteria could grow aerobically. The cells which grew aerobically had oxidase activity. An initial inoculum of 105 cells/ml was used Stationary phase was reached (1.4×109 cells/ml) after 4–5 days growth. When the cells were disrupted using ultrasonication, 2.6 mg bacterial magnetites were obtained from 1 I culture of magnetotactic bacteria. The detection of mouse IgG was carried out using FITC conjugated anti-mouse IgG immobilized on bacterial magnetites and a flow injection system with a fluorescence spectrophotometer. Relative fluorescence intensity correlated linearly with the concentration of mouse IgG in the range from 0.5-100 ng/ml and the measurements were established within 2 min using this system.
AB - Isolated helical shaped magnetotactic bacteria were cultured in a medium containing succinate, nitrate, and ferric malate as carbon, nitrogen, and iron sources, respectively. The magnetotactic bacteria could grow aerobically. The cells which grew aerobically had oxidase activity. An initial inoculum of 105 cells/ml was used Stationary phase was reached (1.4×109 cells/ml) after 4–5 days growth. When the cells were disrupted using ultrasonication, 2.6 mg bacterial magnetites were obtained from 1 I culture of magnetotactic bacteria. The detection of mouse IgG was carried out using FITC conjugated anti-mouse IgG immobilized on bacterial magnetites and a flow injection system with a fluorescence spectrophotometer. Relative fluorescence intensity correlated linearly with the concentration of mouse IgG in the range from 0.5-100 ng/ml and the measurements were established within 2 min using this system.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025488758&partnerID=8YFLogxK
U2 - 10.1109/20.104444
DO - 10.1109/20.104444
M3 - Article
AN - SCOPUS:0025488758
VL - 26
SP - 1557
EP - 1559
JO - IEEE Transactions on Magnetics
JF - IEEE Transactions on Magnetics
SN - 0018-9464
IS - 5
ER -