Mass spectrometric screening of ligands with lower off-rate from a clicked-based pooled library

Satoshi Arai, Shota Hirosawa, Yusuke Oguchi, Madoka Suzuki, Atsushi Murata, Shin'Ichi Ishiwata, Shinji Takeoka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This paper describes a convenient screening method using ion trap electrospray ionization mass spectrometry to classify ligands to a target molecule in terms of kinetic parameters. We demonstrate this method in the screening of ligands to a hexahistidine tag from a pooled library synthesized by click chemistry. The ion trap mass spectrometry analysis revealed that higher stabilities of ligand-target complexes in the gas phase were related to lower dissociation rate constants, i.e., off-rates in solution. Finally, we prepared a fluorescent probe utilizing the ligand with lowest off-rate and succeeded in performing single molecule observations of hexahistidine-tagged myosin V walking on actin filaments.

Original languageEnglish
Pages (from-to)451-455
Number of pages5
JournalACS Combinatorial Science
Volume14
Issue number8
DOIs
Publication statusPublished - 2012 Aug 13

    Fingerprint

Keywords

  • click chemistry
  • fluorescent probes
  • high-throughput screening
  • mass spectrometry

ASJC Scopus subject areas

  • Chemistry(all)

Cite this