Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host

Hidehiro Toh, Brian L. Weiss, Sarah A H Perkin, Atsushi Yamashita, Kenshiro Oshima, Masahira Hattori, Serap Aksoy

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

Sodalis glossinidius is a maternally transmitted endosymbiont of tsetse flies (Glossina spp.), an insect of medical and veterinary significance. Analysis of the complete sequence of Sodalis' chromosome (4,171,146 bp, encoding 2,432 protein coding sequences) indicates a reduced coding capacity of 51%. Furthermore, the chromosome contains 972 pseudogenes, an inordinately high number compared with that of other bacterial species. A high proportion of these pseudogenes are homologs of known proteins that function either in defense or in the transport and metabolism of carbohydrates and inorganic ions, suggesting Sodalis' degenerative adaptations to the immunity and restricted nutritional status of the host. Sodalis possesses three chromosomal symbiosis regions (SSR): SSR-1, SSR-2, and SSR-3, with gene inventories similar to the Type-III secretion system (TTSS) ysa from Yersinia enterolitica and SPI-1 and SPI-2 from Salmonella, respectively. While core components of the needle structure have been conserved, some of the effectors and regulators typically associated with these systems in pathogenic microbes are modified or eliminated in Sodalis. Analysis of SSR-specific invA transcript abundance in Sodalis during host development indicates that the individual symbiosis regions may exhibit different temporal expression profiles. In addition, the Sodalis chromosome encodes a complete flagella structure, key components of which are expressed in immature host developmental stages. These features may be important for the transmission and establishment of symbiont infections in the intra-uterine progeny. The data suggest that Sodalis represents an evolutionary intermediate transitioning from a free-living to a mutualistic lifestyle.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalGenome Research
Volume16
Issue number2
DOIs
Publication statusPublished - 2006 Feb
Externally publishedYes

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Enterobacteriaceae
Symbiosis
Life Style
Genome
Tsetse Flies
Pseudogenes
Chromosomes
Yersinia
Chromosomes, Human, Pair 4
Flagella
Carbohydrate Metabolism
Nutritional Status
Salmonella
Needles
Sequence Analysis
Insects
Immunity
Proteins
Ions
Equipment and Supplies

ASJC Scopus subject areas

  • Genetics

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Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host. / Toh, Hidehiro; Weiss, Brian L.; Perkin, Sarah A H; Yamashita, Atsushi; Oshima, Kenshiro; Hattori, Masahira; Aksoy, Serap.

In: Genome Research, Vol. 16, No. 2, 02.2006, p. 149-156.

Research output: Contribution to journalArticle

Toh, Hidehiro ; Weiss, Brian L. ; Perkin, Sarah A H ; Yamashita, Atsushi ; Oshima, Kenshiro ; Hattori, Masahira ; Aksoy, Serap. / Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host. In: Genome Research. 2006 ; Vol. 16, No. 2. pp. 149-156.
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