Mathematical modeling of dormant cell formation in growing biofilm

Kotaro Chihara, Shinya Matsumoto, Yuki Kagawa, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Understanding the dynamics of dormant cells in microbial biofilms, in which the bacteria are embedded in extracellular matrix, is important for developing successful antibiotic therapies against pathogenic bacteria. Although some of the molecular mechanisms leading to bacterial persistence have been speculated in planktonic bacterial cell, how dormant cells emerge in the biofilms of pathogenic bacteria such as Pseudomonas aeruginosa remains unclear. The present study proposes four hypotheses of dormant cell formation; stochastic process, nutrient-dependent, oxygen-dependent, and time-dependent processes. These hypotheses were implemented into a three-dimensional individual-based model of biofilm formation. Numerical simulations of the different mechanisms yielded qualitatively different spatiotemporal distributions of dormant cells in the growing biofilm. Based on these simulation results, we discuss what kinds of experimental studies are effective for discriminating dormant cell formation mechanisms in biofilms.

    Original languageEnglish
    Article number534
    JournalFrontiers in Microbiology
    Volume6
    Issue numberMAY
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Biofilms
    Bacteria
    Stochastic Processes
    Pseudomonas aeruginosa
    Extracellular Matrix
    Oxygen
    Anti-Bacterial Agents
    Food

    Keywords

    • Biofilm
    • Dormancy
    • Individual-based modeling
    • Mathematical model
    • Persistence

    ASJC Scopus subject areas

    • Microbiology
    • Microbiology (medical)

    Cite this

    Mathematical modeling of dormant cell formation in growing biofilm. / Chihara, Kotaro; Matsumoto, Shinya; Kagawa, Yuki; Tsuneda, Satoshi.

    In: Frontiers in Microbiology, Vol. 6, No. MAY, 534, 2015.

    Research output: Contribution to journalArticle

    Chihara, Kotaro ; Matsumoto, Shinya ; Kagawa, Yuki ; Tsuneda, Satoshi. / Mathematical modeling of dormant cell formation in growing biofilm. In: Frontiers in Microbiology. 2015 ; Vol. 6, No. MAY.
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