Mathematical modeling of dormant cell formation in growing biofilm

Kotaro Chihara, Shinya Matsumoto, Yuki Kagawa, Satoshi Tsuneda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Understanding the dynamics of dormant cells in microbial biofilms, in which the bacteria are embedded in extracellular matrix, is important for developing successful antibiotic therapies against pathogenic bacteria. Although some of the molecular mechanisms leading to bacterial persistence have been speculated in planktonic bacterial cell, how dormant cells emerge in the biofilms of pathogenic bacteria such as Pseudomonas aeruginosa remains unclear. The present study proposes four hypotheses of dormant cell formation; stochastic process, nutrient-dependent, oxygen-dependent, and time-dependent processes. These hypotheses were implemented into a three-dimensional individual-based model of biofilm formation. Numerical simulations of the different mechanisms yielded qualitatively different spatiotemporal distributions of dormant cells in the growing biofilm. Based on these simulation results, we discuss what kinds of experimental studies are effective for discriminating dormant cell formation mechanisms in biofilms.

Original languageEnglish
Article number534
JournalFrontiers in Microbiology
Volume6
Issue numberMAY
DOIs
Publication statusPublished - 2015

Keywords

  • Biofilm
  • Dormancy
  • Individual-based modeling
  • Mathematical model
  • Persistence

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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