Measurement of effective thermophysical properties for Cryopreserved-State evaluation in frozen red blood cells suspension

Nobusuke Shimamoto, Masanobu Ujihira, Masayuki Suzuki, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

Abstract

The purpose of this study is to estimate the viability of frozen biological materials during cryopreservation with the thermophysical properties. Human red blood cells are selected as test biological material. Propylene glycol (0, 10 and 35%w/w) was selected as cryoprotectant. The test chamber was contacted with carbon heater on a copper block immersed in liquid nitrogen, and cooled from room temperature to -196°C at a cooling rate of 1 to 300°C/min. Temperature rise of 5°C was applied to the suspension at -190°C, the effective thermal diffusivity was determined with Laplace transform method. As a result, the effective thermal diffusivity decreased with decrease of intra-and extracellular ice formation fraction in their suspension, which affects their survival after thawing. Thus assessment of cryopreserved biological materials can be achieved with the thermophysical properties.

Original languageEnglish
Pages (from-to)3068-3076
Number of pages9
JournalNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Volume64
Issue number625
Publication statusPublished - 1998

Fingerprint

thermophysical properties
erythrocytes
thermal diffusivity
Biological materials
Blood
Thermodynamic properties
Thermal diffusivity
Cells
test chambers
ice formation
evaluation
propylene
viability
heaters
liquid nitrogen
glycols
Thawing
Laplace transforms
melting
Liquid nitrogen

Keywords

  • Cryopreservation
  • Freezing
  • Medical engineering
  • Thermophysical properties

ASJC Scopus subject areas

  • Mechanical Engineering
  • Condensed Matter Physics

Cite this

Measurement of effective thermophysical properties for Cryopreserved-State evaluation in frozen red blood cells suspension. / Shimamoto, Nobusuke; Ujihira, Masanobu; Suzuki, Masayuki; Oka, Kotaro; Tanishita, Kazuo.

In: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B, Vol. 64, No. 625, 1998, p. 3068-3076.

Research output: Contribution to journalArticle

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AB - The purpose of this study is to estimate the viability of frozen biological materials during cryopreservation with the thermophysical properties. Human red blood cells are selected as test biological material. Propylene glycol (0, 10 and 35%w/w) was selected as cryoprotectant. The test chamber was contacted with carbon heater on a copper block immersed in liquid nitrogen, and cooled from room temperature to -196°C at a cooling rate of 1 to 300°C/min. Temperature rise of 5°C was applied to the suspension at -190°C, the effective thermal diffusivity was determined with Laplace transform method. As a result, the effective thermal diffusivity decreased with decrease of intra-and extracellular ice formation fraction in their suspension, which affects their survival after thawing. Thus assessment of cryopreserved biological materials can be achieved with the thermophysical properties.

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