Abstract
We measured and the endogenous plasma concentration of thrombopoietin (TPO) in 76 patients with acquired aplastic anaemia (AA) by a sensitive sandwich enzyme-linked immunosorbent assay (ELISA). The plasma TPO concentrations were significantly higher in AA patients when compared to healthy control subjects (P<0.0001) and there was a significant negative correlation between plasma TPO concentrations and platelet counts in 54 AA patients who had not received any platelet transfusions prior to sampling. On the other hand, there was no statistically significant correlation between the TPO concentrations and platelet counts in 22 AA patients who had previously received platelet transfusions. We studied serial changes of plasma TPO concentration in 24 patients showed an increase in their platelet counts following bone marrow transplantation or immunosuppressive (IS) therapy. Although a decrease in plasma TPO concentration as observed, levels remained above the range of normal healthy controls even in the patients who attained normal platelet counts following therapy. A decrease in TPO concentrations was observed in only half of the responders to IS therapy. Whether exogenous TPO will result in increased platelet counts in AA patients with high TPO levels remain to be determined.
| Original language | English |
|---|---|
| Pages (from-to) | 538-543 |
| Number of pages | 6 |
| Journal | British Journal of Haematology |
| Volume | 97 |
| Issue number | 3 |
| Publication status | Published - 1997 |
| Externally published | Yes |
Fingerprint
Keywords
- aplastic anaemia
- bone marrow transplantation
- ELISA
- immunosuppressive therapy
- thrombopoietin
ASJC Scopus subject areas
- Hematology
Cite this
Measurement of endogenous plasma thrombopoietin in patients with acquired aplastic anaemia by a sensitive enzyme-linked immunosorbent assay. / Kojima, Seiji; Matsuyama, Takaharu; Kodera, Yoshihisa; Tahara, Tomoyuki; Kato, Takashi.
In: British Journal of Haematology, Vol. 97, No. 3, 1997, p. 538-543.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of endogenous plasma thrombopoietin in patients with acquired aplastic anaemia by a sensitive enzyme-linked immunosorbent assay
AU - Kojima, Seiji
AU - Matsuyama, Takaharu
AU - Kodera, Yoshihisa
AU - Tahara, Tomoyuki
AU - Kato, Takashi
PY - 1997
Y1 - 1997
N2 - We measured and the endogenous plasma concentration of thrombopoietin (TPO) in 76 patients with acquired aplastic anaemia (AA) by a sensitive sandwich enzyme-linked immunosorbent assay (ELISA). The plasma TPO concentrations were significantly higher in AA patients when compared to healthy control subjects (P<0.0001) and there was a significant negative correlation between plasma TPO concentrations and platelet counts in 54 AA patients who had not received any platelet transfusions prior to sampling. On the other hand, there was no statistically significant correlation between the TPO concentrations and platelet counts in 22 AA patients who had previously received platelet transfusions. We studied serial changes of plasma TPO concentration in 24 patients showed an increase in their platelet counts following bone marrow transplantation or immunosuppressive (IS) therapy. Although a decrease in plasma TPO concentration as observed, levels remained above the range of normal healthy controls even in the patients who attained normal platelet counts following therapy. A decrease in TPO concentrations was observed in only half of the responders to IS therapy. Whether exogenous TPO will result in increased platelet counts in AA patients with high TPO levels remain to be determined.
AB - We measured and the endogenous plasma concentration of thrombopoietin (TPO) in 76 patients with acquired aplastic anaemia (AA) by a sensitive sandwich enzyme-linked immunosorbent assay (ELISA). The plasma TPO concentrations were significantly higher in AA patients when compared to healthy control subjects (P<0.0001) and there was a significant negative correlation between plasma TPO concentrations and platelet counts in 54 AA patients who had not received any platelet transfusions prior to sampling. On the other hand, there was no statistically significant correlation between the TPO concentrations and platelet counts in 22 AA patients who had previously received platelet transfusions. We studied serial changes of plasma TPO concentration in 24 patients showed an increase in their platelet counts following bone marrow transplantation or immunosuppressive (IS) therapy. Although a decrease in plasma TPO concentration as observed, levels remained above the range of normal healthy controls even in the patients who attained normal platelet counts following therapy. A decrease in TPO concentrations was observed in only half of the responders to IS therapy. Whether exogenous TPO will result in increased platelet counts in AA patients with high TPO levels remain to be determined.
KW - aplastic anaemia
KW - bone marrow transplantation
KW - ELISA
KW - immunosuppressive therapy
KW - thrombopoietin
UR - http://www.scopus.com/inward/record.url?scp=8244227980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8244227980&partnerID=8YFLogxK
M3 - Article
VL - 97
SP - 538
EP - 543
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 3
ER -