Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins

Sa Rula, Takahiro Suwa, Saku T. Kijima, Takeshi Haraguchi, Shinryu Wakatsuki, Naruki Sato, Zhongrui Duan, Motoki Tominaga, Taro Q.P. Uyeda, Kohji Ito

Research output: Contribution to journalArticle

Abstract

There are two classes of myosin, XI and VIII, in higher plants. Myosin XI moves actin filaments at high speed and its enzyme activity is also very high. In contrast, myosin VIII moves actin filaments very slowly with very low enzyme activity. Because most of these enzymatic and motile activities were measured using animal skeletal muscle α-actin, but not plant actin, they would not accurately reflect the actual activities in plant cells. We thus measured enzymatic and motile activities of the motor domains of two Arabidopsis myosin XI isoforms (MYA2, XI-B), and one Arabidopsis myosin VIII isoform (ATM1), by using three Arabidopsis actin isoforms (ACT1, ACT2, and ACT7). The measured activities were different from those measured by using muscle actin. Moreover, Arabidopsis myosins showed different enzymatic and motile activities when using different Arabidopsis actin isoforms. Our results suggest that plant actin should be used for measuring enzymatic and motile activities of plant myosins and that different actin isoforms in plant cells might function as different tracks along which affinities and velocities of each myosin isoform are modulated.

Original languageEnglish
Pages (from-to)2145-2151
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume495
Issue number3
DOIs
Publication statusPublished - 2018 Jan 15

Fingerprint

Myosins
Arabidopsis
Actins
Protein Isoforms
Plant Cells
Actin Cytoskeleton
Enzyme activity
Muscle
Enzymes
Skeletal Muscle
Motor Activity
Animals
Muscles

Keywords

  • Actin
  • Arabidopsis
  • Cytoplasmic streaming
  • Myosin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins. / Rula, Sa; Suwa, Takahiro; Kijima, Saku T.; Haraguchi, Takeshi; Wakatsuki, Shinryu; Sato, Naruki; Duan, Zhongrui; Tominaga, Motoki; Uyeda, Taro Q.P.; Ito, Kohji.

In: Biochemical and Biophysical Research Communications, Vol. 495, No. 3, 15.01.2018, p. 2145-2151.

Research output: Contribution to journalArticle

Rula, S, Suwa, T, Kijima, ST, Haraguchi, T, Wakatsuki, S, Sato, N, Duan, Z, Tominaga, M, Uyeda, TQP & Ito, K 2018, 'Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins', Biochemical and Biophysical Research Communications, vol. 495, no. 3, pp. 2145-2151. https://doi.org/10.1016/j.bbrc.2017.12.071
Rula, Sa ; Suwa, Takahiro ; Kijima, Saku T. ; Haraguchi, Takeshi ; Wakatsuki, Shinryu ; Sato, Naruki ; Duan, Zhongrui ; Tominaga, Motoki ; Uyeda, Taro Q.P. ; Ito, Kohji. / Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 495, No. 3. pp. 2145-2151.
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