Measurement of incident angle dependence of swimming bacterium reflection using on-chip single-cell cultivation assay

Akihiro Hattori, Senkei Umehara, Yuichi Wakamoto, Kenji Yasuda

Research output: Contribution to journalLetter

8 Citations (Scopus)

Abstract

We have developed an on-chip single-cell microcultivation assay as a means of continuously observing certain single swimming cells in order to trace their movement. The single cells were captured in microchambers fabricated on thin glass slides and having semipermeable membrane lids, in which cells can swim within the space for a long term without escaping. This assay enables the direct measurement of the reflection of certain cells against the microchamber walls depending on their incidence angles. Using this assay, the reflection was examined. We found that the ratio of reflection of cells to those of non- reverse was almost the same, though most of cells reflected when their incident angle was perpendicular to the wall.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume42
Issue number7 B
Publication statusPublished - 2003 Jul 15
Externally publishedYes

Fingerprint

bacteria
Assays
Bacteria
chips
cells
Membranes
Glass
Swimming
chutes
incidence
membranes
glass

Keywords

  • Bacteria
  • Incident angle
  • Microchamber
  • Microcultivation
  • Movement
  • Reflection
  • Single cell

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

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title = "Measurement of incident angle dependence of swimming bacterium reflection using on-chip single-cell cultivation assay",
abstract = "We have developed an on-chip single-cell microcultivation assay as a means of continuously observing certain single swimming cells in order to trace their movement. The single cells were captured in microchambers fabricated on thin glass slides and having semipermeable membrane lids, in which cells can swim within the space for a long term without escaping. This assay enables the direct measurement of the reflection of certain cells against the microchamber walls depending on their incidence angles. Using this assay, the reflection was examined. We found that the ratio of reflection of cells to those of non- reverse was almost the same, though most of cells reflected when their incident angle was perpendicular to the wall.",
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journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",
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TY - JOUR

T1 - Measurement of incident angle dependence of swimming bacterium reflection using on-chip single-cell cultivation assay

AU - Hattori, Akihiro

AU - Umehara, Senkei

AU - Wakamoto, Yuichi

AU - Yasuda, Kenji

PY - 2003/7/15

Y1 - 2003/7/15

N2 - We have developed an on-chip single-cell microcultivation assay as a means of continuously observing certain single swimming cells in order to trace their movement. The single cells were captured in microchambers fabricated on thin glass slides and having semipermeable membrane lids, in which cells can swim within the space for a long term without escaping. This assay enables the direct measurement of the reflection of certain cells against the microchamber walls depending on their incidence angles. Using this assay, the reflection was examined. We found that the ratio of reflection of cells to those of non- reverse was almost the same, though most of cells reflected when their incident angle was perpendicular to the wall.

AB - We have developed an on-chip single-cell microcultivation assay as a means of continuously observing certain single swimming cells in order to trace their movement. The single cells were captured in microchambers fabricated on thin glass slides and having semipermeable membrane lids, in which cells can swim within the space for a long term without escaping. This assay enables the direct measurement of the reflection of certain cells against the microchamber walls depending on their incidence angles. Using this assay, the reflection was examined. We found that the ratio of reflection of cells to those of non- reverse was almost the same, though most of cells reflected when their incident angle was perpendicular to the wall.

KW - Bacteria

KW - Incident angle

KW - Microchamber

KW - Microcultivation

KW - Movement

KW - Reflection

KW - Single cell

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M3 - Letter

AN - SCOPUS:0141606678

VL - 42

JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

SN - 0021-4922

IS - 7 B

ER -