Measurement of inflammatory cytokine secretion from human monocytes after inflammasome activation

Yoshitaka Shirasaki, Asahi Nakahara, Nanako Shimura, Kazushi Izawa, Nobutake Suzuki, Mai Yamagishi, Jun Mizuno, Tetsushi Sekiguchi, Ryuta Nishikomori, Shuichi Shoji, Osamu Ohara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. Some of the lipopolysaccharide primed monocytes secreted small amounts of interleukin-1 beta (IL-1 ), whereas the monocytes stimulated with an ATP signal secreted large amounts of IL-1 from 10% of observed cells. These two types of secretion patterns corresponded to the proposed models of IL-1 secretion, "rescue and redirect" and "terminal release", respectively. Morphological changes in ATP-stimulated cells also indicated that the cells underwent necrotic cell death, also known as "pyroptosis".

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1012-1014
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CityOkinawa
Period12/10/2812/11/1

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Keywords

  • Cytokine secretion
  • Inflammation
  • Monocyte
  • Single cell

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Shirasaki, Y., Nakahara, A., Shimura, N., Izawa, K., Suzuki, N., Yamagishi, M., Mizuno, J., Sekiguchi, T., Nishikomori, R., Shoji, S., & Ohara, O. (2012). Measurement of inflammatory cytokine secretion from human monocytes after inflammasome activation. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1012-1014). Chemical and Biological Microsystems Society.