Measurement of photosynthesis activity using single synecocystis SP. PCC 6803 on microchambers having gas barrier wall and fluorescence oxygen sensor

Hisataka Maruyama, yu Matsuda, Tomohide Niimi, Nobuyuki Unozumi, Kei Nanatani, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier wall. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier wall to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation method for photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.

Original languageEnglish
Title of host publication2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
Pages476-478
Number of pages3
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes
Event23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012 - Nagoya, Japan
Duration: 2012 Nov 42012 Nov 7

Other

Other23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012
CountryJapan
CityNagoya
Period12/11/412/11/7

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering

Cite this

Maruyama, H., Matsuda, Y., Niimi, T., Unozumi, N., Nanatani, K., & Arai, F. (2012). Measurement of photosynthesis activity using single synecocystis SP. PCC 6803 on microchambers having gas barrier wall and fluorescence oxygen sensor. In 2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012 (pp. 476-478). [6492497] https://doi.org/10.1109/MHS.2012.6492497