Measurement of single low-density lipoprotein particles by atomic force microscopy

Toshihiro Sakurai, Seiji Takeda, Jun Ya Takahashi, Yuji Takahashi, Norio Wada, Suchin Trirongjitmoah, Takeshi Namita, Shigeki Jin, Akiko Ikuta, Hiroaki Furumaki, Shu Ping Hui, Hirotoshi Fuda, Masato Fujikawa, Koichi Shimizu, Hitoshi Chiba

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P <0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P <0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P <0.05 for large LDL and P <0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.

Original languageEnglish
Pages (from-to)564-570
Number of pages7
JournalAnnals of Clinical Biochemistry
Volume50
Issue number6
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Atomic Force Microscopy
LDL Lipoproteins
Atomic force microscopy
Particle Size
Electron microscopy
Electron Microscopy
Particle size
Lipoproteins
Coronary Artery Disease
Ultracentrifugation
Healthy Volunteers
oxidized low density lipoprotein

Keywords

  • AFM
  • Electron microscopy
  • Large LDL
  • Small dense LDL

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Sakurai, T., Takeda, S., Takahashi, J. Y., Takahashi, Y., Wada, N., Trirongjitmoah, S., ... Chiba, H. (2013). Measurement of single low-density lipoprotein particles by atomic force microscopy. Annals of Clinical Biochemistry, 50(6), 564-570. https://doi.org/10.1177/0004563213481586

Measurement of single low-density lipoprotein particles by atomic force microscopy. / Sakurai, Toshihiro; Takeda, Seiji; Takahashi, Jun Ya; Takahashi, Yuji; Wada, Norio; Trirongjitmoah, Suchin; Namita, Takeshi; Jin, Shigeki; Ikuta, Akiko; Furumaki, Hiroaki; Hui, Shu Ping; Fuda, Hirotoshi; Fujikawa, Masato; Shimizu, Koichi; Chiba, Hitoshi.

In: Annals of Clinical Biochemistry, Vol. 50, No. 6, 2013, p. 564-570.

Research output: Contribution to journalArticle

Sakurai, T, Takeda, S, Takahashi, JY, Takahashi, Y, Wada, N, Trirongjitmoah, S, Namita, T, Jin, S, Ikuta, A, Furumaki, H, Hui, SP, Fuda, H, Fujikawa, M, Shimizu, K & Chiba, H 2013, 'Measurement of single low-density lipoprotein particles by atomic force microscopy', Annals of Clinical Biochemistry, vol. 50, no. 6, pp. 564-570. https://doi.org/10.1177/0004563213481586
Sakurai T, Takeda S, Takahashi JY, Takahashi Y, Wada N, Trirongjitmoah S et al. Measurement of single low-density lipoprotein particles by atomic force microscopy. Annals of Clinical Biochemistry. 2013;50(6):564-570. https://doi.org/10.1177/0004563213481586
Sakurai, Toshihiro ; Takeda, Seiji ; Takahashi, Jun Ya ; Takahashi, Yuji ; Wada, Norio ; Trirongjitmoah, Suchin ; Namita, Takeshi ; Jin, Shigeki ; Ikuta, Akiko ; Furumaki, Hiroaki ; Hui, Shu Ping ; Fuda, Hirotoshi ; Fujikawa, Masato ; Shimizu, Koichi ; Chiba, Hitoshi. / Measurement of single low-density lipoprotein particles by atomic force microscopy. In: Annals of Clinical Biochemistry. 2013 ; Vol. 50, No. 6. pp. 564-570.
@article{8c7a177ebc164c5c921aba2e4b7a175b,
title = "Measurement of single low-density lipoprotein particles by atomic force microscopy",
abstract = "Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P <0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P <0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P <0.05 for large LDL and P <0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.",
keywords = "AFM, Electron microscopy, Large LDL, Small dense LDL",
author = "Toshihiro Sakurai and Seiji Takeda and Takahashi, {Jun Ya} and Yuji Takahashi and Norio Wada and Suchin Trirongjitmoah and Takeshi Namita and Shigeki Jin and Akiko Ikuta and Hiroaki Furumaki and Hui, {Shu Ping} and Hirotoshi Fuda and Masato Fujikawa and Koichi Shimizu and Hitoshi Chiba",
year = "2013",
doi = "10.1177/0004563213481586",
language = "English",
volume = "50",
pages = "564--570",
journal = "Annals of Clinical Biochemistry",
issn = "0004-5632",
publisher = "SAGE Publications Ltd",
number = "6",

}

TY - JOUR

T1 - Measurement of single low-density lipoprotein particles by atomic force microscopy

AU - Sakurai, Toshihiro

AU - Takeda, Seiji

AU - Takahashi, Jun Ya

AU - Takahashi, Yuji

AU - Wada, Norio

AU - Trirongjitmoah, Suchin

AU - Namita, Takeshi

AU - Jin, Shigeki

AU - Ikuta, Akiko

AU - Furumaki, Hiroaki

AU - Hui, Shu Ping

AU - Fuda, Hirotoshi

AU - Fujikawa, Masato

AU - Shimizu, Koichi

AU - Chiba, Hitoshi

PY - 2013

Y1 - 2013

N2 - Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P <0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P <0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P <0.05 for large LDL and P <0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.

AB - Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P <0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P <0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P <0.05 for large LDL and P <0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.

KW - AFM

KW - Electron microscopy

KW - Large LDL

KW - Small dense LDL

UR - http://www.scopus.com/inward/record.url?scp=84899528411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899528411&partnerID=8YFLogxK

U2 - 10.1177/0004563213481586

DO - 10.1177/0004563213481586

M3 - Article

VL - 50

SP - 564

EP - 570

JO - Annals of Clinical Biochemistry

JF - Annals of Clinical Biochemistry

SN - 0004-5632

IS - 6

ER -