Measurement of the bio-mechanical properties of two different feeder layer cells

S. Romanazzo, K. Uesugi, A. Taniguchi, G. Forte, K. Morishima

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: We here present our findings on 2 types of feeder layers, one composed of mouse embryonic fibroblasts (MEF) and the second one of mouse skeletal myoblasts (C2Cl2) feeder cells. Methods: The 2 feeder layers present a dramatic variance of intrinsic stiffness (142.68 ± 17.21 KPa and 45.78 ± 9.81 KPa, respectively). Results and Conclusion: This information could be used for a better understanding of cells and cell microenvironment mechano-physical characteristics that are influencing stem cell commitment, in order to develop a suitable engineered tissue for cardiac and skeletal muscle repair and a bio-actuator.

Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalOpen Biotechnology Journal
Volume15
Issue number1
DOIs
Publication statusPublished - 2021

Keywords

  • Bio-actuator
  • Bio-mechanical properties
  • Cell sheet
  • Mouse embryonic fibroblasts (MEF)
  • Mouse skeletal myoblast
  • Skeletal tensile test

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biochemistry
  • Biomedical Engineering

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