Measurement of the Cell Migration and Cytokine Secretion Activity with Real-Time Imaging System

Yumiko Tanaka, Nobutake Suzuki, Kazuyo Moro, Jun Mizuno, Shuichi Shoji, Sotaro Uemura, Yoshitaka Shirasaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Immune cells communicate with each other to regulate their activity. Cytokines are humoral proteins secreted and received by specific cells as one of the communication media. Although several single-cell techniques to measure the cytokine secretion have been reported, it is still hard to simultaneously monitor the secretion activity and motility of individual cells composing a cell community. Previously, we have developed the platform of wide-field LCI-S (live-cell imaging for secretion activity) with the open-spaced cell culture chamber on the optical waveguide. We have monitored type2 cytokine secretion and motility from hundreds of ILC2s (Group 2 innate lymphoid cells). Here, we conducted a detailed analysis of the obtained data to reveal the relevance between cell migration patterns and cytokine secretion dynamics of migrating cells. We tracked each cell on bright field images and found two types of cell migration features: directional and random motilities. We also estimated the production amount of the secreted cytokine at each time point by calculating the serial cumulative images of secreted cytokine. By combining these data, we succeeded in examining the relationship between motility and secretion activity. These results demonstrated that wide-field LCI-S has the potential to elucidate immune cells' behavior as a cell population that is regulated by cell-To-cell communication via humoral factors.

Original languageEnglish
Title of host publicationMHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538667927
DOIs
Publication statusPublished - 2018 Dec
Event29th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2018 - Nagoya, Japan
Duration: 2018 Dec 102018 Dec 12

Publication series

NameMHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science

Conference

Conference29th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2018
CountryJapan
CityNagoya
Period18/12/1018/12/12

ASJC Scopus subject areas

  • Human-Computer Interaction
  • Artificial Intelligence
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Control and Optimization
  • Instrumentation

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  • Cite this

    Tanaka, Y., Suzuki, N., Moro, K., Mizuno, J., Shoji, S., Uemura, S., & Shirasaki, Y. (2018). Measurement of the Cell Migration and Cytokine Secretion Activity with Real-Time Imaging System. In MHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science [8887045] (MHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2018.8887045