Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse

Yuta Sekiguchi, Kazuto Masamoto, Hiroyuki Takuwa, Hiroshi Kawaguchi, Iwao Kanno, Hiroshi Ito, Yutaka Tomita, Yoshiaki Itoh, Norihiro Suzuki, Ryo Sudo, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)

Abstract

The present study reports a semiautomatic image analysis method for measuring the spatiotemporal dynamics of the vessel dilation that was fluorescently imaged with either confocal or two-photon microscope. With this method, arterial dilation induced by whisker stimulation was compared between cortical surface and parenchymal tissue in the vibrissae area of somatosensory cortex in awake Tie2-GFP mice in which the vascular endothelium had genetically expressed green fluorescent protein. We observed that a mean arterial diameter during a pre-stimulus baseline state was 39 ± 7, 19 ± 1, 16 ± 4, 17 ± 4, and 14 ± 3 μm at depths of 0, 100, 200, 300, and 400 μm, respectively. The stimulation-evoked dilation induced by mechanical whisker deflection (10 Hz for 5 s) was 3.4 ± 0.8, 1.8 ± 0.8, 1.8 ± 0.9, 1.6 ± 0.9, and 1.5 ± 0.6 μm at each depth, respectively. Consequently, no significant differences were observed for the vessel dilation rate between the cortical surface and parenchymal arteries: 8.8 %, 9.9 %, 10.9 %, 9.2 %, and 10.3 % relative to their baseline diameters, respectively. These preliminary results demonstrate that the present method is useful to further investigate the quantitative relationships between the spatiotemporally varying arterial tone and the associated blood flow changes in the parenchymal microcirculation to reveal the regulatory mechanism of the cerebral blood flow.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages419-425
Number of pages7
Volume789
ISBN (Print)9781461472568
DOIs
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume789
ISSN (Print)00652598

Fingerprint

Vibrissae
Blood Vessels
Dilatation
Brain
Blood
Arteries
Microcirculation
Green Fluorescent Proteins
Image analysis
Cerebrovascular Circulation
Microscopes
Photons
Tissue
Somatosensory Cortex
Vascular Endothelium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sekiguchi, Y., Masamoto, K., Takuwa, H., Kawaguchi, H., Kanno, I., Ito, H., ... Tanishita, K. (2013). Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. In Advances in Experimental Medicine and Biology (Vol. 789, pp. 419-425). (Advances in Experimental Medicine and Biology; Vol. 789). Springer New York LLC. https://doi.org/10.1007/978-1-4614-7411-1_56

Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. / Sekiguchi, Yuta; Masamoto, Kazuto; Takuwa, Hiroyuki; Kawaguchi, Hiroshi; Kanno, Iwao; Ito, Hiroshi; Tomita, Yutaka; Itoh, Yoshiaki; Suzuki, Norihiro; Sudo, Ryo; Tanishita, Kazuo.

Advances in Experimental Medicine and Biology. Vol. 789 Springer New York LLC, 2013. p. 419-425 (Advances in Experimental Medicine and Biology; Vol. 789).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sekiguchi, Y, Masamoto, K, Takuwa, H, Kawaguchi, H, Kanno, I, Ito, H, Tomita, Y, Itoh, Y, Suzuki, N, Sudo, R & Tanishita, K 2013, Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. in Advances in Experimental Medicine and Biology. vol. 789, Advances in Experimental Medicine and Biology, vol. 789, Springer New York LLC, pp. 419-425. https://doi.org/10.1007/978-1-4614-7411-1_56
Sekiguchi Y, Masamoto K, Takuwa H, Kawaguchi H, Kanno I, Ito H et al. Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. In Advances in Experimental Medicine and Biology. Vol. 789. Springer New York LLC. 2013. p. 419-425. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4614-7411-1_56
Sekiguchi, Yuta ; Masamoto, Kazuto ; Takuwa, Hiroyuki ; Kawaguchi, Hiroshi ; Kanno, Iwao ; Ito, Hiroshi ; Tomita, Yutaka ; Itoh, Yoshiaki ; Suzuki, Norihiro ; Sudo, Ryo ; Tanishita, Kazuo. / Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. Advances in Experimental Medicine and Biology. Vol. 789 Springer New York LLC, 2013. pp. 419-425 (Advances in Experimental Medicine and Biology).
@inproceedings{345f3f3ef4a543319089721aed8e6949,
title = "Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse",
abstract = "The present study reports a semiautomatic image analysis method for measuring the spatiotemporal dynamics of the vessel dilation that was fluorescently imaged with either confocal or two-photon microscope. With this method, arterial dilation induced by whisker stimulation was compared between cortical surface and parenchymal tissue in the vibrissae area of somatosensory cortex in awake Tie2-GFP mice in which the vascular endothelium had genetically expressed green fluorescent protein. We observed that a mean arterial diameter during a pre-stimulus baseline state was 39 ± 7, 19 ± 1, 16 ± 4, 17 ± 4, and 14 ± 3 μm at depths of 0, 100, 200, 300, and 400 μm, respectively. The stimulation-evoked dilation induced by mechanical whisker deflection (10 Hz for 5 s) was 3.4 ± 0.8, 1.8 ± 0.8, 1.8 ± 0.9, 1.6 ± 0.9, and 1.5 ± 0.6 μm at each depth, respectively. Consequently, no significant differences were observed for the vessel dilation rate between the cortical surface and parenchymal arteries: 8.8 {\%}, 9.9 {\%}, 10.9 {\%}, 9.2 {\%}, and 10.3 {\%} relative to their baseline diameters, respectively. These preliminary results demonstrate that the present method is useful to further investigate the quantitative relationships between the spatiotemporally varying arterial tone and the associated blood flow changes in the parenchymal microcirculation to reveal the regulatory mechanism of the cerebral blood flow.",
author = "Yuta Sekiguchi and Kazuto Masamoto and Hiroyuki Takuwa and Hiroshi Kawaguchi and Iwao Kanno and Hiroshi Ito and Yutaka Tomita and Yoshiaki Itoh and Norihiro Suzuki and Ryo Sudo and Kazuo Tanishita",
year = "2013",
doi = "10.1007/978-1-4614-7411-1_56",
language = "English",
isbn = "9781461472568",
volume = "789",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer New York LLC",
pages = "419--425",
booktitle = "Advances in Experimental Medicine and Biology",

}

TY - GEN

T1 - Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse

AU - Sekiguchi, Yuta

AU - Masamoto, Kazuto

AU - Takuwa, Hiroyuki

AU - Kawaguchi, Hiroshi

AU - Kanno, Iwao

AU - Ito, Hiroshi

AU - Tomita, Yutaka

AU - Itoh, Yoshiaki

AU - Suzuki, Norihiro

AU - Sudo, Ryo

AU - Tanishita, Kazuo

PY - 2013

Y1 - 2013

N2 - The present study reports a semiautomatic image analysis method for measuring the spatiotemporal dynamics of the vessel dilation that was fluorescently imaged with either confocal or two-photon microscope. With this method, arterial dilation induced by whisker stimulation was compared between cortical surface and parenchymal tissue in the vibrissae area of somatosensory cortex in awake Tie2-GFP mice in which the vascular endothelium had genetically expressed green fluorescent protein. We observed that a mean arterial diameter during a pre-stimulus baseline state was 39 ± 7, 19 ± 1, 16 ± 4, 17 ± 4, and 14 ± 3 μm at depths of 0, 100, 200, 300, and 400 μm, respectively. The stimulation-evoked dilation induced by mechanical whisker deflection (10 Hz for 5 s) was 3.4 ± 0.8, 1.8 ± 0.8, 1.8 ± 0.9, 1.6 ± 0.9, and 1.5 ± 0.6 μm at each depth, respectively. Consequently, no significant differences were observed for the vessel dilation rate between the cortical surface and parenchymal arteries: 8.8 %, 9.9 %, 10.9 %, 9.2 %, and 10.3 % relative to their baseline diameters, respectively. These preliminary results demonstrate that the present method is useful to further investigate the quantitative relationships between the spatiotemporally varying arterial tone and the associated blood flow changes in the parenchymal microcirculation to reveal the regulatory mechanism of the cerebral blood flow.

AB - The present study reports a semiautomatic image analysis method for measuring the spatiotemporal dynamics of the vessel dilation that was fluorescently imaged with either confocal or two-photon microscope. With this method, arterial dilation induced by whisker stimulation was compared between cortical surface and parenchymal tissue in the vibrissae area of somatosensory cortex in awake Tie2-GFP mice in which the vascular endothelium had genetically expressed green fluorescent protein. We observed that a mean arterial diameter during a pre-stimulus baseline state was 39 ± 7, 19 ± 1, 16 ± 4, 17 ± 4, and 14 ± 3 μm at depths of 0, 100, 200, 300, and 400 μm, respectively. The stimulation-evoked dilation induced by mechanical whisker deflection (10 Hz for 5 s) was 3.4 ± 0.8, 1.8 ± 0.8, 1.8 ± 0.9, 1.6 ± 0.9, and 1.5 ± 0.6 μm at each depth, respectively. Consequently, no significant differences were observed for the vessel dilation rate between the cortical surface and parenchymal arteries: 8.8 %, 9.9 %, 10.9 %, 9.2 %, and 10.3 % relative to their baseline diameters, respectively. These preliminary results demonstrate that the present method is useful to further investigate the quantitative relationships between the spatiotemporally varying arterial tone and the associated blood flow changes in the parenchymal microcirculation to reveal the regulatory mechanism of the cerebral blood flow.

UR - http://www.scopus.com/inward/record.url?scp=84934438525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934438525&partnerID=8YFLogxK

U2 - 10.1007/978-1-4614-7411-1_56

DO - 10.1007/978-1-4614-7411-1_56

M3 - Conference contribution

AN - SCOPUS:84934438525

SN - 9781461472568

VL - 789

T3 - Advances in Experimental Medicine and Biology

SP - 419

EP - 425

BT - Advances in Experimental Medicine and Biology

PB - Springer New York LLC

ER -