Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy

Hatsuki Shiga, Yukako Yamane, Etsuro Ito, Kazuhiro Abe, Kazushige Kawabata, Hisashi Haga

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to examine the mechanical properties of the membrane surface of astrocytes, we observed living astrocytes by atomic force microscopy (AFM) both in contact mode and force-mapping mode. Ridge-like structures reflecting actin filaments were observed in the topographic images in contact mode, but not in force-mapping mode, using a zero-loading force. When we measured the elasticity of astrocytes, we observed that the cell membrane above the nucleus was soft and the cell membrane above the cytosol was stiff. In particular, the parts reflecting actin filaments were very stiff. This effect of actin filaments on the elasticity of astrocytes was confirmed by the loss of actin filaments after application of actin-polymerization inhibitor.

Original languageEnglish
Pages (from-to)3711-3716
Number of pages6
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume39
Issue number6 B
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Atomic force microscopy
filaments
atomic force microscopy
mechanical properties
membranes
Membranes
Mechanical properties
Cell membranes
Elasticity
elastic properties
inhibitors
ridges
polymerization
Polymerization
nuclei
Astrocytes

Keywords

  • Actin filament
  • Astrocyte
  • Atomic force microscopy
  • Cell membrane
  • Elasticity
  • Force mapping
  • Young's modulus

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy. / Shiga, Hatsuki; Yamane, Yukako; Ito, Etsuro; Abe, Kazuhiro; Kawabata, Kazushige; Haga, Hisashi.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 39, No. 6 B, 2000, p. 3711-3716.

Research output: Contribution to journalArticle

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AU - Haga, Hisashi

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