Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Takayuki Akimoto; Takashi Ushida; Shigeru Miyaki; Hiroshi Akaogi; Kohei Tsuchiya; Zhen Yan; R. Sanders Williams; Tetsuya Tateishi

doi:10.1016/j.bbrc.2005.01.136

Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Takayuki Akimoto^*, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Kohei Tsuchiya, Zhen Yan, R. Sanders Williams, Tetsuya Tateishi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

Original language	English
Pages (from-to)	381-385
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	329
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.01.136
Publication status	Published - 2005 Apr 1
Externally published	Yes

Keywords

Adipocytes
C2C12
Mechanical stress
Mechanical stretch
Myoblasts
Trans-differentiation
Wnt10

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2005.01.136

Cite this

@article{1c35ecc03df24e2fb83c915ca293bf6f,

title = "Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling",

abstract = "Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.",

keywords = "Adipocytes, C2C12, Mechanical stress, Mechanical stretch, Myoblasts, Trans-differentiation, Wnt10",

author = "Takayuki Akimoto and Takashi Ushida and Shigeru Miyaki and Hiroshi Akaogi and Kohei Tsuchiya and Zhen Yan and Williams, {R. Sanders} and Tetsuya Tateishi",

note = "Funding Information: We are grateful to Dr. Takashi Sato and Dr. Ping Li for critical comments on the manuscript. This work was supported by a Grant-in-Aid from National Institute of Advanced Industrial Sciences and Technology (AIST), and National Institute of Material Sciences (NIMS), Japan. Takayuki Akimoto was the recipient of a Grant-in Aid for Overseas Research Scholars from the Ministry of Education, Science and Culture of Japan.",

year = "2005",

month = apr,

day = "1",

doi = "10.1016/j.bbrc.2005.01.136",

language = "English",

volume = "329",

pages = "381--385",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

AU - Akimoto, Takayuki

AU - Ushida, Takashi

AU - Miyaki, Shigeru

AU - Akaogi, Hiroshi

AU - Tsuchiya, Kohei

AU - Yan, Zhen

AU - Williams, R. Sanders

AU - Tateishi, Tetsuya

N1 - Funding Information: We are grateful to Dr. Takashi Sato and Dr. Ping Li for critical comments on the manuscript. This work was supported by a Grant-in-Aid from National Institute of Advanced Industrial Sciences and Technology (AIST), and National Institute of Material Sciences (NIMS), Japan. Takayuki Akimoto was the recipient of a Grant-in Aid for Overseas Research Scholars from the Ministry of Education, Science and Culture of Japan.

PY - 2005/4/1

Y1 - 2005/4/1

N2 - Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

AB - Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

KW - Adipocytes

KW - C2C12

KW - Mechanical stress

KW - Mechanical stretch

KW - Myoblasts

KW - Trans-differentiation

KW - Wnt10

UR - http://www.scopus.com/inward/record.url?scp=13844281666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844281666&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.01.136

DO - 10.1016/j.bbrc.2005.01.136

M3 - Article

C2 - 15721317

AN - SCOPUS:13844281666

SN - 0006-291X

VL - 329

SP - 381

EP - 385

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this