Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Takayuki Akimoto, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Kohei Tsuchiya, Zhen Yan, R. Sanders Williams, Tetsuya Tateishi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number1
DOIs
Publication statusPublished - 2005 Apr 1
Externally publishedYes

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Myoblasts
Adipocytes
Osteoblasts
Elongation
Animals
Ligands
Messenger RNA
Adipogenesis

Keywords

  • Adipocytes
  • C2C12
  • Mechanical stress
  • Mechanical stretch
  • Myoblasts
  • Trans-differentiation
  • Wnt10

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling. / Akimoto, Takayuki; Ushida, Takashi; Miyaki, Shigeru; Akaogi, Hiroshi; Tsuchiya, Kohei; Yan, Zhen; Williams, R. Sanders; Tateishi, Tetsuya.

In: Biochemical and Biophysical Research Communications, Vol. 329, No. 1, 01.04.2005, p. 381-385.

Research output: Contribution to journalArticle

Akimoto, Takayuki ; Ushida, Takashi ; Miyaki, Shigeru ; Akaogi, Hiroshi ; Tsuchiya, Kohei ; Yan, Zhen ; Williams, R. Sanders ; Tateishi, Tetsuya. / Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 329, No. 1. pp. 381-385.
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