Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Takayuki Akimoto, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Kohei Tsuchiya, Zhen Yan, R. Sanders Williams, Tetsuya Tateishi

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number1
DOIs
Publication statusPublished - 2005 Apr 1
Externally publishedYes

Keywords

  • Adipocytes
  • C2C12
  • Mechanical stress
  • Mechanical stretch
  • Myoblasts
  • Trans-differentiation
  • Wnt10

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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