Mechanism of autocatalytic oxidation of oxyhemoglobin by nitrite

H. Kosaka, I. Tyuma, K. Imaizumi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Oxidation of oxyhemoglobin by nitrite is characterized by the presence of a lag phase followed by the autocatalysis. In phosphate buffer, an asymmetric ESR signal is detected at g = 2.005 (hereafter referred to as the g = 2 radical) during the oxidation which is similar to that of the methemoglobin free radical generated from methemoglobin and H2O2. Catalase and KCN prolong the oxidation, indicating the involvement of H2O2 and methemoglobin in the reaction. Superoxide dismutase, on the other hand, does not modify the oxidation. The present results suggest a chain reaction mechanism for the oxidation in which the g = 2 radical catalyzes the formation of NO2 from NO2 - by a peroxidase action and NO2 oxidizes oxyhemoglobin. However, in N,N-bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane (bistris) buffer, superoxide dismutase markedly elongates the lag phase and accelerates the autocatalysis: bistris scavenges the g = 2 radical and a radical derived from bistris reduces O2 to O2 -.

Original languageEnglish
JournalBiomedica Biochimica Acta
Volume42
Issue number11-12
Publication statusPublished - 1983
Externally publishedYes

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Methemoglobin
Oxyhemoglobins
Nitrites
Oxidation
Superoxide Dismutase
Buffers
Methane
Catalase
Peroxidase
Free Radicals
Phosphates
Paramagnetic resonance
Bistris

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kosaka, H., Tyuma, I., & Imaizumi, K. (1983). Mechanism of autocatalytic oxidation of oxyhemoglobin by nitrite. Biomedica Biochimica Acta, 42(11-12).

Mechanism of autocatalytic oxidation of oxyhemoglobin by nitrite. / Kosaka, H.; Tyuma, I.; Imaizumi, K.

In: Biomedica Biochimica Acta, Vol. 42, No. 11-12, 1983.

Research output: Contribution to journalArticle

Kosaka, H, Tyuma, I & Imaizumi, K 1983, 'Mechanism of autocatalytic oxidation of oxyhemoglobin by nitrite', Biomedica Biochimica Acta, vol. 42, no. 11-12.
Kosaka, H. ; Tyuma, I. ; Imaizumi, K. / Mechanism of autocatalytic oxidation of oxyhemoglobin by nitrite. In: Biomedica Biochimica Acta. 1983 ; Vol. 42, No. 11-12.
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