Mechanism of flocculate formation of highly concentrated phospholipid vesicles suspended in a series of water-soluble biopolymers

Hiromi Sakai, Atsushi Sato, Shinji Takeoka, Eishun Tsuchida

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Polyethylene glycol-modified vesicles (liposomes) encapsulating hemoglobin (HbV) are artificial oxygen carriers that have been developed as a transfusion alternative. The HbV suspension in an albumin solution is nearly Newtonian; other biopolymers, hydroxyethyl starch (HES), dextran (DEX), and modified fluid gelatin, induce flocculation of HbVs through depletion interaction and render the suspensions as non-Newtonian. The flocculation level increased with hydrodynamic radius (Rh) or radius of gyration (Rg) of series of HES or DEX with different molecular weights at a constant polymer concentration (4 wt %). However, the flocculation level differed markedly among the polymers. A crowding index (Ci) representing the crowding level of a polymer solution is defined as (excluded volume of one polymer) x (molar concentration) x Avogadro's number, using Rh or Rg. All polymers' flocculation level increases when Ci approaches 1: when the theoretical total excluded volumes approach the entire solution volume, the excluded HbV particles are forced to flocculate.

    Original languageEnglish
    Pages (from-to)2344-2350
    Number of pages7
    JournalBiomacromolecules
    Volume10
    Issue number8
    DOIs
    Publication statusPublished - 2009 Aug 10

    Fingerprint

    Biopolymers
    Phospholipids
    Flocculation
    Polymers
    Dextran
    Water
    Dextrans
    Starch
    Suspensions
    Blood Substitutes
    Liposomes
    Hemoglobin
    Polymer solutions
    Polyethylene glycols
    Albumins
    Hydrodynamics
    Molecular weight
    Oxygen
    Fluids

    ASJC Scopus subject areas

    • Bioengineering
    • Materials Chemistry
    • Polymers and Plastics
    • Biomaterials

    Cite this

    Mechanism of flocculate formation of highly concentrated phospholipid vesicles suspended in a series of water-soluble biopolymers. / Sakai, Hiromi; Sato, Atsushi; Takeoka, Shinji; Tsuchida, Eishun.

    In: Biomacromolecules, Vol. 10, No. 8, 10.08.2009, p. 2344-2350.

    Research output: Contribution to journalArticle

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