Mechanism of glucose oxidase immobilization with silk fibroin

Tetsuo Asakura, Hiroaki Yoshimizu, Akio Kuzuhara, Tadashi Matsunaga

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Magnetic resonance spectroscopy, NMR and ESR, was applied for the examination of the interaction between glucose oxidase (GOD) and Bombyx mori silk fibroin, in order to analyze the superior attributes of silk fibroin for the immobilization of GOD. The 13C NMR spectra and spin-lattice relaxation times, T1, of B, mori silk fibroin in aqueous solution did not change in the presence of GOD. The spin-labeled silk fibroin membrane immobilizing GOD gave also the same ESR spectrum as that of the GODfree membrane. In the presence of silk fibroin, the rate of the GOD reaction in aqueous solution monitored by 13C NMR slightly decreased, although no peak broadening occurred, indicating a slight decrease in the diffusion of glucose and, as a result, a slight decrease in the contact between glucose and GOD rather than silk fibroin inhibition. In addition, no appreciable interaction was observed between the silk fibroin and 14 kinds of amino acids in the 13C NMR spectra.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalJournal of Sericultural Science of Japan
Volume57
Issue number3
DOIs
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Fibroins
fibroins
glucose oxidase
Glucose Oxidase
Glucose oxidase
Silk
silk
Immobilization
Nuclear magnetic resonance
aqueous solutions
Paramagnetic resonance
Glucose
Magnetic resonance spectroscopy
Membranes
Enzyme inhibition
Morus
glucose
Bombyx
Spin-lattice relaxation
Bombyx mori

ASJC Scopus subject areas

  • Materials Science(all)
  • Polymers and Plastics

Cite this

Mechanism of glucose oxidase immobilization with silk fibroin. / Asakura, Tetsuo; Yoshimizu, Hiroaki; Kuzuhara, Akio; Matsunaga, Tadashi.

In: Journal of Sericultural Science of Japan, Vol. 57, No. 3, 1988, p. 203-209.

Research output: Contribution to journalArticle

Asakura, Tetsuo ; Yoshimizu, Hiroaki ; Kuzuhara, Akio ; Matsunaga, Tadashi. / Mechanism of glucose oxidase immobilization with silk fibroin. In: Journal of Sericultural Science of Japan. 1988 ; Vol. 57, No. 3. pp. 203-209.
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