Megakaryocyte growth and development factor: Analyses of in vitro effects on human megakaryopoiesis and endogenous serum levels during chemotherapy-induced thrombocytopenia

Janet L. Nichol, Martha M. Hokom, Alex Hornkohl, William P. Sheridan, Hideya Ohashi, Takashi Kato, Yue Sheng Li, Timothy D. Bartley, Esther Choi, Jakob Bogenberger, James D. Skrine, Andrew Knudten, Janice Chen, Geri Trail, Lisa Sleeman, Sean Cole, Gustavo Grampp, Pamela Hunt

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

The present study shows that recombinant human megakaryocyte growth and development factor (r-HuMGDF) behaves both as a megakaryocyte colony stimulating factor and as a differentiation factor in human progenitor cell cultures. Megakaryocyte colony formation induced with r-HuMGDF is synergistically affected by stem cell factor but not by interleukin 3. Megakaryocytes stimulated with r-HuMGDF demonstrate progressive cytoplasmic and nuclear maturation. Measurable levels of megakaryocyte growth and development factor in serum from patients undergoing myeloablative therapy and transplantation are shown to be elaborated in response to thrombocytopenic stress. These data support the concept that megakaryocyte growth and development factor is a physiologically regulated cytokine that is capable of supporting several aspects of megakaryopoiesis.

Original languageEnglish
Pages (from-to)2973-2978
Number of pages6
JournalJournal of Clinical Investigation
Volume95
Issue number6
Publication statusPublished - 1995 Jun
Externally publishedYes

Fingerprint

Thrombopoietin
Thrombocytopenia
Statistical Factor Analysis
Drug Therapy
Serum
Megakaryocytes
Stem Cell Factor
Interleukin-3
In Vitro Techniques
Stem Cells
Cell Culture Techniques
Transplantation
Cytokines

Keywords

  • Colony forming unit-megakaryocyte
  • Cytokine
  • Patient serum
  • Ploidy
  • Thrombopoietin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Megakaryocyte growth and development factor : Analyses of in vitro effects on human megakaryopoiesis and endogenous serum levels during chemotherapy-induced thrombocytopenia. / Nichol, Janet L.; Hokom, Martha M.; Hornkohl, Alex; Sheridan, William P.; Ohashi, Hideya; Kato, Takashi; Li, Yue Sheng; Bartley, Timothy D.; Choi, Esther; Bogenberger, Jakob; Skrine, James D.; Knudten, Andrew; Chen, Janice; Trail, Geri; Sleeman, Lisa; Cole, Sean; Grampp, Gustavo; Hunt, Pamela.

In: Journal of Clinical Investigation, Vol. 95, No. 6, 06.1995, p. 2973-2978.

Research output: Contribution to journalArticle

Nichol, JL, Hokom, MM, Hornkohl, A, Sheridan, WP, Ohashi, H, Kato, T, Li, YS, Bartley, TD, Choi, E, Bogenberger, J, Skrine, JD, Knudten, A, Chen, J, Trail, G, Sleeman, L, Cole, S, Grampp, G & Hunt, P 1995, 'Megakaryocyte growth and development factor: Analyses of in vitro effects on human megakaryopoiesis and endogenous serum levels during chemotherapy-induced thrombocytopenia', Journal of Clinical Investigation, vol. 95, no. 6, pp. 2973-2978.
Nichol, Janet L. ; Hokom, Martha M. ; Hornkohl, Alex ; Sheridan, William P. ; Ohashi, Hideya ; Kato, Takashi ; Li, Yue Sheng ; Bartley, Timothy D. ; Choi, Esther ; Bogenberger, Jakob ; Skrine, James D. ; Knudten, Andrew ; Chen, Janice ; Trail, Geri ; Sleeman, Lisa ; Cole, Sean ; Grampp, Gustavo ; Hunt, Pamela. / Megakaryocyte growth and development factor : Analyses of in vitro effects on human megakaryopoiesis and endogenous serum levels during chemotherapy-induced thrombocytopenia. In: Journal of Clinical Investigation. 1995 ; Vol. 95, No. 6. pp. 2973-2978.
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