Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8

Tsukasa Higuchi, Kenichi Koike, Nobukuni Sawai, Hadija Hemed Mwamtemi, Kouichi Takeuchi, Masaaki Shiohara, Toshimi Kikuchi, Kozo Yasui, Susumu Ito, Osamu Yamagami, Yumiko Sasaki, Nobuo Okumura, Takashi Kato, Hiroshi Miyazaki, Masayuki Ikeda, Muneo Yamada, Atsushi Komiyama

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14 Citations (Scopus)

Abstract

In a serum-free liquid culture, thrombopoietin (TPD) selectively stimulated the growth of megakaryocytic cells from CD34-positive cord blood cells. Using these cultured cells, we investigated cytokine production by human megakaryocytes. Day 10 megakaryocytes (2 x 105) secreted > 1000 pg/ml of interleukin (IL)-8, in contrast to small amounts of IL-1β and IL-6. A time-course study showed that the IL-8 production of megakaryocytes occurred at the late phase of the culture period. The megakaryocyte-conditioned medium had the chemotactic potential of polymorphonuclear leucocytes, which was abrogated by the addition of anti-IL-8 antibody, suggesting the secretion of biologically active IL-8. The combination of TPO and IL-1α was required for a significant augmentation of the IL-8 secretion. Direct evidence for IL-8 synthesis in megakaryocytes was provided by reverse transcription-polymerase chain reaction on purified CD41b+ cells and by the detection of intracellular IL-8 in CD41b+ cells. These results suggest that TPO stimulates not only the proliferation and differentiation of the progenitors capable of megakaryocytic lineage expression but also IL-8 release by the megakaryocytic cells with the aid of IL-1.

Original languageEnglish
Pages (from-to)509-516
Number of pages8
JournalBritish Journal of Haematology
Volume99
Issue number3
DOIs
Publication statusPublished - 1997

Keywords

  • Cord blood
  • IL-1
  • IL-8 production
  • Megakaryocyte
  • TPO

ASJC Scopus subject areas

  • Hematology

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    Higuchi, T., Koike, K., Sawai, N., Mwamtemi, H. H., Takeuchi, K., Shiohara, M., Kikuchi, T., Yasui, K., Ito, S., Yamagami, O., Sasaki, Y., Okumura, N., Kato, T., Miyazaki, H., Ikeda, M., Yamada, M., & Komiyama, A. (1997). Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8. British Journal of Haematology, 99(3), 509-516. https://doi.org/10.1046/j.1365-2141.1997.4253232.x