Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8

Tsukasa Higuchi, Kenichi Koike, Nobukuni Sawai, Hadija Hemed Mwamtemi, Kouichi Takeuchi, Masaaki Shiohara, Toshimi Kikuchi, Kozo Yasui, Susumu Ito, Osamu Yamagami, Yumiko Sasaki, Nobuo Okumura, Takashi Kato, Hiroshi Miyazaki, Masayuki Ikeda, Muneo Yamada, Atsushi Komiyama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In a serum-free liquid culture, thrombopoietin (TPD) selectively stimulated the growth of megakaryocytic cells from CD34-positive cord blood cells. Using these cultured cells, we investigated cytokine production by human megakaryocytes. Day 10 megakaryocytes (2 x 105) secreted > 1000 pg/ml of interleukin (IL)-8, in contrast to small amounts of IL-1β and IL-6. A time-course study showed that the IL-8 production of megakaryocytes occurred at the late phase of the culture period. The megakaryocyte-conditioned medium had the chemotactic potential of polymorphonuclear leucocytes, which was abrogated by the addition of anti-IL-8 antibody, suggesting the secretion of biologically active IL-8. The combination of TPO and IL-1α was required for a significant augmentation of the IL-8 secretion. Direct evidence for IL-8 synthesis in megakaryocytes was provided by reverse transcription-polymerase chain reaction on purified CD41b+ cells and by the detection of intracellular IL-8 in CD41b+ cells. These results suggest that TPO stimulates not only the proliferation and differentiation of the progenitors capable of megakaryocytic lineage expression but also IL-8 release by the megakaryocytic cells with the aid of IL-1.

Original languageEnglish
Pages (from-to)509-516
Number of pages8
JournalBritish Journal of Haematology
Volume99
Issue number3
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Megakaryocytes
Interleukin-8
Fetal Blood
Blood Cells
Interleukin-1
Thrombopoietin
Conditioned Culture Medium
Reverse Transcription
Cultured Cells
Interleukin-6
Neutrophils
Cytokines
Polymerase Chain Reaction
Antibodies
Growth
Serum

Keywords

  • Cord blood
  • IL-1
  • IL-8 production
  • Megakaryocyte
  • TPO

ASJC Scopus subject areas

  • Hematology

Cite this

Higuchi, T., Koike, K., Sawai, N., Mwamtemi, H. H., Takeuchi, K., Shiohara, M., ... Komiyama, A. (1997). Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8. British Journal of Haematology, 99(3), 509-516.

Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8. / Higuchi, Tsukasa; Koike, Kenichi; Sawai, Nobukuni; Mwamtemi, Hadija Hemed; Takeuchi, Kouichi; Shiohara, Masaaki; Kikuchi, Toshimi; Yasui, Kozo; Ito, Susumu; Yamagami, Osamu; Sasaki, Yumiko; Okumura, Nobuo; Kato, Takashi; Miyazaki, Hiroshi; Ikeda, Masayuki; Yamada, Muneo; Komiyama, Atsushi.

In: British Journal of Haematology, Vol. 99, No. 3, 1997, p. 509-516.

Research output: Contribution to journalArticle

Higuchi, T, Koike, K, Sawai, N, Mwamtemi, HH, Takeuchi, K, Shiohara, M, Kikuchi, T, Yasui, K, Ito, S, Yamagami, O, Sasaki, Y, Okumura, N, Kato, T, Miyazaki, H, Ikeda, M, Yamada, M & Komiyama, A 1997, 'Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8', British Journal of Haematology, vol. 99, no. 3, pp. 509-516.
Higuchi T, Koike K, Sawai N, Mwamtemi HH, Takeuchi K, Shiohara M et al. Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8. British Journal of Haematology. 1997;99(3):509-516.
Higuchi, Tsukasa ; Koike, Kenichi ; Sawai, Nobukuni ; Mwamtemi, Hadija Hemed ; Takeuchi, Kouichi ; Shiohara, Masaaki ; Kikuchi, Toshimi ; Yasui, Kozo ; Ito, Susumu ; Yamagami, Osamu ; Sasaki, Yumiko ; Okumura, Nobuo ; Kato, Takashi ; Miyazaki, Hiroshi ; Ikeda, Masayuki ; Yamada, Muneo ; Komiyama, Atsushi. / Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8. In: British Journal of Haematology. 1997 ; Vol. 99, No. 3. pp. 509-516.
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