Mesocylindrical aluminosilica monolith biocaptors for size-selective macromolecule cargos

S. A. El-Safty, M. A. Shenashen, M. Ismael, M. Khairy

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    Immobilization of biological macromolecules, such as protein, onto solid supports is an important method for diagnostic assays andgenetechnology. This present study reports the size-selective adsorption/removal of virtual proteins that have different shapes, sizes, functions, and properties, such as insulin, cytochrome c, lysozyme, myoglobin, β-lactoglobin, α-amylase, hemoglobin, and myosin in aqueous water using mesobiocaptor monoliths. To prevent large proteins from adsorbing and remaining attached to adsorbent surfaces, large, open, cylindrical-pored, three-dimensional cubic aluminosilica mesostructures with large aluminum contents and micrometer-sized monolith particles were fabricated. The unique physical properties and the surface functionality of the mesobiocaptors enhance protein adsorption characteristics in terms of loading capacity and quantity of the sample, ensuring a higher concentration of adsorbed proteins, interior pore diffusivity, and encapsulation in a short period. Thermodynamic studies indicate that protein adsorption into the mesobiocaptor pores is favorable and spontaneous. Theoretical models were used to investigate the major driving forces for the most optimal performance of the protein adsorption. The geometrical findings point to key factors, such as surface energy, intermolecular forces, charge distribution, hydrophobicity, and electrostatic interaction, which might control the adsorption into the interior large, open cylindrical mesobiocaptor cavities (sized 3-16 nm) without aggregation of these proteins on the exterior surfaces of monoliths. Indeed, the availability of adsorption of single proteins from mixtures based on size- and shape-selective separation opens new avenues of research in encapsulation of proteins and bioanalysis.

    Original languageEnglish
    Pages (from-to)3013-3021
    Number of pages9
    JournalAdvanced Functional Materials
    Volume22
    Issue number14
    DOIs
    Publication statusPublished - 2012 Jul 24

    Fingerprint

    Macromolecules
    macromolecules
    proteins
    Proteins
    Adsorption
    adsorption
    Encapsulation
    Porins
    porosity
    myosins
    Myoglobin
    Charge distribution
    insulin
    myoglobin
    intermolecular forces
    Myosins
    Amylases
    Hydrophobicity
    Muramidase
    Cytochromes c

    Keywords

    • 3D geometrical models
    • biocaptors
    • cargo proteins
    • encapsulation
    • mesocylindrical

    ASJC Scopus subject areas

    • Biomaterials
    • Electrochemistry
    • Condensed Matter Physics
    • Electronic, Optical and Magnetic Materials

    Cite this

    Mesocylindrical aluminosilica monolith biocaptors for size-selective macromolecule cargos. / El-Safty, S. A.; Shenashen, M. A.; Ismael, M.; Khairy, M.

    In: Advanced Functional Materials, Vol. 22, No. 14, 24.07.2012, p. 3013-3021.

    Research output: Contribution to journalArticle

    El-Safty, S. A. ; Shenashen, M. A. ; Ismael, M. ; Khairy, M. / Mesocylindrical aluminosilica monolith biocaptors for size-selective macromolecule cargos. In: Advanced Functional Materials. 2012 ; Vol. 22, No. 14. pp. 3013-3021.
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