Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector

Chuanbo Gao, Isabel Izquierdo-Barba, Ikuhiko Nakase, Shiroh Futaki, Juanfang Ruan, Kazutami Sakamoto, Yasuhiro Sakamoto, Kazuyuki Kuroda, Osamu Terasaki, Shunai Che

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A drug delivery system using mesostructured silica as a reservoir has been developed for the storage and controlled release of a drug with a cell-penetrating peptide (CPP) as a vector. We use fluorescein isothiocyanate (FITC) as the drug model and octaarginine (R8) as a vector to endow the drug with cell-penetrating property. The mesostructured silica reservoir system was prepared by using a one-pot liquid-crystal templating method, which is suitable for the encapsulation of intact FITC-R8 conjugates and sustained release of drugs without hampering their properties. The hydrophobic poly(propyl oxide) (PPO) shell of the pore-filling Pluronic F127 and the electrostatic interaction between R8 and siloxide ions on the pore walls act as the diffusion-limiting factors of the FITC-R8 conjugate. A sigmoidal in vitro release of FITC-R8 from mesostructured silica into phosphate buffered saline (PBS, pH 7.4) was observed and the typical release duration was 5 days at 37 °C. Release from the reservoir yielded significant elongation in duration of the FITC signals in DU145 cells by confocal microscopic analysis, compared with a single administration of FITC-R8.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalMicroporous and Mesoporous Materials
Volume122
Issue number1-3
DOIs
Publication statusPublished - 2009 Jun 1

Keywords

  • Cell-penetrating peptide
  • Drug delivery
  • Mesostructured silica
  • Sigmoidal release
  • Sustained cellular uptake

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials

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  • Cite this

    Gao, C., Izquierdo-Barba, I., Nakase, I., Futaki, S., Ruan, J., Sakamoto, K., Sakamoto, Y., Kuroda, K., Terasaki, O., & Che, S. (2009). Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector. Microporous and Mesoporous Materials, 122(1-3), 201-207. https://doi.org/10.1016/j.micromeso.2009.03.002