Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector

Chuanbo Gao, Isabel Izquierdo-Barba, Ikuhiko Nakase, Shiroh Futaki, Juanfang Ruan, Kazutami Sakamoto, Yasuhiro Sakamoto, Kazuyuki Kuroda, Osamu Terasaki, Shunai Che

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    A drug delivery system using mesostructured silica as a reservoir has been developed for the storage and controlled release of a drug with a cell-penetrating peptide (CPP) as a vector. We use fluorescein isothiocyanate (FITC) as the drug model and octaarginine (R8) as a vector to endow the drug with cell-penetrating property. The mesostructured silica reservoir system was prepared by using a one-pot liquid-crystal templating method, which is suitable for the encapsulation of intact FITC-R8 conjugates and sustained release of drugs without hampering their properties. The hydrophobic poly(propyl oxide) (PPO) shell of the pore-filling Pluronic F127 and the electrostatic interaction between R8 and siloxide ions on the pore walls act as the diffusion-limiting factors of the FITC-R8 conjugate. A sigmoidal in vitro release of FITC-R8 from mesostructured silica into phosphate buffered saline (PBS, pH 7.4) was observed and the typical release duration was 5 days at 37 °C. Release from the reservoir yielded significant elongation in duration of the FITC signals in DU145 cells by confocal microscopic analysis, compared with a single administration of FITC-R8.

    Original languageEnglish
    Pages (from-to)201-207
    Number of pages7
    JournalMicroporous and Mesoporous Materials
    Volume122
    Issue number1-3
    DOIs
    Publication statusPublished - 2009 Jun 1

    Fingerprint

    Fluorescein
    Silicon Dioxide
    Peptides
    peptides
    delivery
    drugs
    Silica
    silicon dioxide
    cells
    Pharmaceutical Preparations
    UCON 50-HB-5100
    Coulomb interactions
    Encapsulation
    Liquid crystals
    Elongation
    Phosphates
    porosity
    Cell-Penetrating Peptides
    Poloxamer
    Liquid Crystals

    Keywords

    • Cell-penetrating peptide
    • Drug delivery
    • Mesostructured silica
    • Sigmoidal release
    • Sustained cellular uptake

    ASJC Scopus subject areas

    • Materials Science(all)
    • Mechanics of Materials
    • Chemistry(all)
    • Condensed Matter Physics

    Cite this

    Gao, C., Izquierdo-Barba, I., Nakase, I., Futaki, S., Ruan, J., Sakamoto, K., ... Che, S. (2009). Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector. Microporous and Mesoporous Materials, 122(1-3), 201-207. https://doi.org/10.1016/j.micromeso.2009.03.002

    Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector. / Gao, Chuanbo; Izquierdo-Barba, Isabel; Nakase, Ikuhiko; Futaki, Shiroh; Ruan, Juanfang; Sakamoto, Kazutami; Sakamoto, Yasuhiro; Kuroda, Kazuyuki; Terasaki, Osamu; Che, Shunai.

    In: Microporous and Mesoporous Materials, Vol. 122, No. 1-3, 01.06.2009, p. 201-207.

    Research output: Contribution to journalArticle

    Gao, C, Izquierdo-Barba, I, Nakase, I, Futaki, S, Ruan, J, Sakamoto, K, Sakamoto, Y, Kuroda, K, Terasaki, O & Che, S 2009, 'Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector', Microporous and Mesoporous Materials, vol. 122, no. 1-3, pp. 201-207. https://doi.org/10.1016/j.micromeso.2009.03.002
    Gao, Chuanbo ; Izquierdo-Barba, Isabel ; Nakase, Ikuhiko ; Futaki, Shiroh ; Ruan, Juanfang ; Sakamoto, Kazutami ; Sakamoto, Yasuhiro ; Kuroda, Kazuyuki ; Terasaki, Osamu ; Che, Shunai. / Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector. In: Microporous and Mesoporous Materials. 2009 ; Vol. 122, No. 1-3. pp. 201-207.
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