Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector

Chuanbo Gao, Isabel Izquierdo-Barba, Ikuhiko Nakase, Shiroh Futaki, Juanfang Ruan, Kazutami Sakamoto, Yasuhiro Sakamoto, Kazuyuki Kuroda, Osamu Terasaki, Shunai Che

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    25 Citations (Scopus)

    Abstract

    A drug delivery system using mesostructured silica as a reservoir has been developed for the storage and controlled release of a drug with a cell-penetrating peptide (CPP) as a vector. We use fluorescein isothiocyanate (FITC) as the drug model and octaarginine (R8) as a vector to endow the drug with cell-penetrating property. The mesostructured silica reservoir system was prepared by using a one-pot liquid-crystal templating method, which is suitable for the encapsulation of intact FITC-R8 conjugates and sustained release of drugs without hampering their properties. The hydrophobic poly(propyl oxide) (PPO) shell of the pore-filling Pluronic F127 and the electrostatic interaction between R8 and siloxide ions on the pore walls act as the diffusion-limiting factors of the FITC-R8 conjugate. A sigmoidal in vitro release of FITC-R8 from mesostructured silica into phosphate buffered saline (PBS, pH 7.4) was observed and the typical release duration was 5 days at 37 °C. Release from the reservoir yielded significant elongation in duration of the FITC signals in DU145 cells by confocal microscopic analysis, compared with a single administration of FITC-R8.

    Original languageEnglish
    Pages (from-to)201-207
    Number of pages7
    JournalMicroporous and Mesoporous Materials
    Volume122
    Issue number1-3
    DOIs
    Publication statusPublished - 2009 Jun 1

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    Keywords

    • Cell-penetrating peptide
    • Drug delivery
    • Mesostructured silica
    • Sigmoidal release
    • Sustained cellular uptake

    ASJC Scopus subject areas

    • Materials Science(all)
    • Mechanics of Materials
    • Chemistry(all)
    • Condensed Matter Physics

    Cite this

    Gao, C., Izquierdo-Barba, I., Nakase, I., Futaki, S., Ruan, J., Sakamoto, K., Sakamoto, Y., Kuroda, K., Terasaki, O., & Che, S. (2009). Mesostructured silica based delivery system for a drug with a peptide as a cell-penetrating vector. Microporous and Mesoporous Materials, 122(1-3), 201-207. https://doi.org/10.1016/j.micromeso.2009.03.002