Meta-analysis of fecal microbiota and metabolites in experimental colitic mice during the inflammatory and healing phases

Toshifumi Osaka, Eri Moriyama, Shunichi Arai, Yasuhiro Date, Junji Yagi, Jun Kikuchi, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The imbalance of gut microbiota is known to be associated with inflammatory bowel disease, but it remains unknown whether dysbiosis is a cause or consequence of chronic gut inflammation. In order to investigate the effects of gut inflammation on microbiota and metabolome, the sequential changes in gut microbiota and metabolites from the onset of colitis to the recovery in dextran sulfate sodium-induced colitic mice were characterized by using meta 16S rRNA sequencing and proton nuclear magnetic resonance (1H-NMR) analysis. Mice in the colitis progression phase showed the transient expansions of two bacterial families including Bacteroidaceae and Enterobacteriaceae and the depletion of major gut commensal bacteria belonging to the uncultured Bacteroidales family S24-7, Rikenellaceae, Lachnospiraceae, and Ruminococcaceae. After the initiation of the recovery, commensal Lactobacillus members promptly predominated in gut while other normally abundant bacteria excluding the Erysipelotrichaceae remained diminished. Furthermore, 1H-NMR analysis revealed characteristic fluctuations in fecal levels of organic acids (lactate and succinate) associated with the disease states. In conclusion, acute intestinal inflammation is a perturbation factor of gut microbiota but alters the intestinal environments suitable for Lactobacillus members.

    Original languageEnglish
    Article number1329
    JournalNutrients
    Volume9
    Issue number12
    DOIs
    Publication statusPublished - 2017 Dec 6

    Fingerprint

    Microbiota
    meta-analysis
    Meta-Analysis
    digestive system
    intestinal microorganisms
    Lactobacillus
    Colitis
    metabolites
    Inflammation
    Bacteroidaceae
    inflammation
    colitis
    mice
    Dysbiosis
    Bacteria
    Dextran Sulfate
    Metabolome
    Succinic Acid
    Enterobacteriaceae
    Inflammatory Bowel Diseases

    Keywords

    • 1H-NMR analysis
    • Dysbiosis
    • Experimental colitic mice
    • Gut microbiota
    • Inflammatory bowel disease
    • Lactobacillus
    • Meta 16S rRNA analysis
    • Metabolome

    ASJC Scopus subject areas

    • Food Science
    • Nutrition and Dietetics

    Cite this

    Meta-analysis of fecal microbiota and metabolites in experimental colitic mice during the inflammatory and healing phases. / Osaka, Toshifumi; Moriyama, Eri; Arai, Shunichi; Date, Yasuhiro; Yagi, Junji; Kikuchi, Jun; Tsuneda, Satoshi.

    In: Nutrients, Vol. 9, No. 12, 1329, 06.12.2017.

    Research output: Contribution to journalArticle

    Osaka, Toshifumi ; Moriyama, Eri ; Arai, Shunichi ; Date, Yasuhiro ; Yagi, Junji ; Kikuchi, Jun ; Tsuneda, Satoshi. / Meta-analysis of fecal microbiota and metabolites in experimental colitic mice during the inflammatory and healing phases. In: Nutrients. 2017 ; Vol. 9, No. 12.
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