Metabolic assay system for micropatterned contractile myotubes

K. Nagamine, Y. Ido, S. Sekine, Takeo Miyake, M. Kanzaki, M. Nisizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Contractile C2C12 myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.

Original languageEnglish
Title of host publicationChemical Sensors 9 -and- MEMS/NEMS 9
Pages35-39
Number of pages5
Volume33
Edition8
DOIs
Publication statusPublished - 2010
Externally publishedYes
EventChemical Sensors 9: Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 9 - 218th ECS Meeting - Las Vegas, NV, United States
Duration: 2010 Oct 102010 Oct 15

Other

OtherChemical Sensors 9: Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 9 - 218th ECS Meeting
CountryUnited States
CityLas Vegas, NV
Period10/10/1010/10/15

Fingerprint

Assays
Gels
Bioassay
Glucose
Proteins

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Nagamine, K., Ido, Y., Sekine, S., Miyake, T., Kanzaki, M., & Nisizawa, M. (2010). Metabolic assay system for micropatterned contractile myotubes. In Chemical Sensors 9 -and- MEMS/NEMS 9 (8 ed., Vol. 33, pp. 35-39) https://doi.org/10.1149/1.3484104

Metabolic assay system for micropatterned contractile myotubes. / Nagamine, K.; Ido, Y.; Sekine, S.; Miyake, Takeo; Kanzaki, M.; Nisizawa, M.

Chemical Sensors 9 -and- MEMS/NEMS 9. Vol. 33 8. ed. 2010. p. 35-39.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nagamine, K, Ido, Y, Sekine, S, Miyake, T, Kanzaki, M & Nisizawa, M 2010, Metabolic assay system for micropatterned contractile myotubes. in Chemical Sensors 9 -and- MEMS/NEMS 9. 8 edn, vol. 33, pp. 35-39, Chemical Sensors 9: Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 9 - 218th ECS Meeting, Las Vegas, NV, United States, 10/10/10. https://doi.org/10.1149/1.3484104
Nagamine K, Ido Y, Sekine S, Miyake T, Kanzaki M, Nisizawa M. Metabolic assay system for micropatterned contractile myotubes. In Chemical Sensors 9 -and- MEMS/NEMS 9. 8 ed. Vol. 33. 2010. p. 35-39 https://doi.org/10.1149/1.3484104
Nagamine, K. ; Ido, Y. ; Sekine, S. ; Miyake, Takeo ; Kanzaki, M. ; Nisizawa, M. / Metabolic assay system for micropatterned contractile myotubes. Chemical Sensors 9 -and- MEMS/NEMS 9. Vol. 33 8. ed. 2010. pp. 35-39
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