Metabolic assay system for micropatterned contractile myotubes

K. Nagamine, Y. Ido, S. Sekine, T. Miyake, M. Kanzaki, M. Nisizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Contractile C2C12 myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.

Original languageEnglish
Title of host publicationChemical Sensors 9 -and- MEMS/NEMS 9
Pages35-39
Number of pages5
Edition8
DOIs
Publication statusPublished - 2010 Dec 1
Externally publishedYes
EventChemical Sensors 9: Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 9 - 218th ECS Meeting - Las Vegas, NV, United States
Duration: 2010 Oct 102010 Oct 15

Publication series

NameECS Transactions
Number8
Volume33
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherChemical Sensors 9: Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 9 - 218th ECS Meeting
CountryUnited States
CityLas Vegas, NV
Period10/10/1010/10/15

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Nagamine, K., Ido, Y., Sekine, S., Miyake, T., Kanzaki, M., & Nisizawa, M. (2010). Metabolic assay system for micropatterned contractile myotubes. In Chemical Sensors 9 -and- MEMS/NEMS 9 (8 ed., pp. 35-39). (ECS Transactions; Vol. 33, No. 8). https://doi.org/10.1149/1.3484104