Abstract
Round spermatids (steps 1–8) were isolated from rat testes and glucose transport into the cells was examined. The exposure of spermatids to glucose resulted in an extremely low level of ATP. In contrast, the level of ATP remained constant in the presence of pyruvate. Transport of a glucose analogue, 2‐deoxy‐D‐[3H]glucose ([3H]dGlc) into spermatids was correlated with intracellular levels of ATP and was much greater in cells with higher rather than lower levels of ATP. [3H]dGlc transport into spermatids with low levels of ATP was partially reversed when the cells were incubated with pyruvate. Inhibition of [3H]dGlc transport was exerted on Vmax and not on Km. Moreover, glucose acted as a competitive inhibitor of [3H]dGlc uptake (Km increased; Vmax unaltered). These results suggest that glucose transport into spermatids is active in vitro and probably regulated by the intracellular level of ATP.
| Original language | English |
|---|---|
| Pages (from-to) | 499-504 |
| Number of pages | 6 |
| Journal | Development Growth and Differentiation |
| Volume | 28 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Metabolism of Round Spermatids : A Possible Regulation of Hexose Transport: hexose transport/ATP/rat spermatids. / NAKAMURA, MASAHISA; YAMAGUCHI, KEIICHIRO; SUZUKI, AKIRA; OKINAGA, SHOICHI; ARAI, KIYOSHI.
In: Development Growth and Differentiation, Vol. 28, No. 5, 1986, p. 499-504.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolism of Round Spermatids
T2 - A Possible Regulation of Hexose Transport: hexose transport/ATP/rat spermatids
AU - NAKAMURA, MASAHISA
AU - YAMAGUCHI, KEIICHIRO
AU - SUZUKI, AKIRA
AU - OKINAGA, SHOICHI
AU - ARAI, KIYOSHI
PY - 1986
Y1 - 1986
N2 - Round spermatids (steps 1–8) were isolated from rat testes and glucose transport into the cells was examined. The exposure of spermatids to glucose resulted in an extremely low level of ATP. In contrast, the level of ATP remained constant in the presence of pyruvate. Transport of a glucose analogue, 2‐deoxy‐D‐[3H]glucose ([3H]dGlc) into spermatids was correlated with intracellular levels of ATP and was much greater in cells with higher rather than lower levels of ATP. [3H]dGlc transport into spermatids with low levels of ATP was partially reversed when the cells were incubated with pyruvate. Inhibition of [3H]dGlc transport was exerted on Vmax and not on Km. Moreover, glucose acted as a competitive inhibitor of [3H]dGlc uptake (Km increased; Vmax unaltered). These results suggest that glucose transport into spermatids is active in vitro and probably regulated by the intracellular level of ATP.
AB - Round spermatids (steps 1–8) were isolated from rat testes and glucose transport into the cells was examined. The exposure of spermatids to glucose resulted in an extremely low level of ATP. In contrast, the level of ATP remained constant in the presence of pyruvate. Transport of a glucose analogue, 2‐deoxy‐D‐[3H]glucose ([3H]dGlc) into spermatids was correlated with intracellular levels of ATP and was much greater in cells with higher rather than lower levels of ATP. [3H]dGlc transport into spermatids with low levels of ATP was partially reversed when the cells were incubated with pyruvate. Inhibition of [3H]dGlc transport was exerted on Vmax and not on Km. Moreover, glucose acted as a competitive inhibitor of [3H]dGlc uptake (Km increased; Vmax unaltered). These results suggest that glucose transport into spermatids is active in vitro and probably regulated by the intracellular level of ATP.
UR - http://www.scopus.com/inward/record.url?scp=84985823952&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84985823952&partnerID=8YFLogxK
U2 - 10.1111/j.1440-169X.1986.00499.x
DO - 10.1111/j.1440-169X.1986.00499.x
M3 - Article
AN - SCOPUS:84985823952
VL - 28
SP - 499
EP - 504
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
SN - 0012-1592
IS - 5
ER -