Glucose was utilized at a rate of 17.9 ± 1.0 nmol · 106 cells-1 · h-1, with 5.2 and 9.5% accumulated as glucose and fructose 1,6-diphosphate, respectively, 79.6% transformed into lactate, 2% oxidized by the trichloroacetic acid cycle, and little metabolized by the pentose cycle. Glucose utilization increased and reached a maximum at 1 mM glucose. The ATP level was maintained by approximately 0.8 mM lactate, but not by glucose or pyruvate. Glucose decreased the ATP level to a value lower than the control level due partly to the fructose 6-phosphate-fructose 1,6-disphosphate futile cycle. In the presence of latate (0.01-20 mM), pyruvate production was increased, but the intracellular level of pyruvate (0.2-0.3 mM) remained unchanged. Lactate dehydrogenase (LDH) activity was increased, at a maximum rate, by 0.1-0.2 mM pyruvate but was inhibited beyond 0.3 mM [K(i) = 2.5 mM]. The K(m) values of LDH for pyruvate and lactate were 24.4 ± 4.2 μM and 2.36 ± 0.14 mM, respectively. The importance of LDH in glucose and lactate metabolism by spermatids was described. These observations further support the hypothesis that lactate is the preferred substrate for spermatid metabolism.
|Journal||American Journal of Physiology - Endocrinology and Metabolism|
|Publication status||Published - 1984|
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