Metal salts requisite for the production of eicosapentaenoic acid by a marine bacterium isolated from mackerel intestines

Masamichi Akimoto, Tetsuya Ishii, Koji Yamagaki, Kazuhisa Ohtaguchi, Kozo Koide, Kazunaga Yazawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Metal salts important for the growth and 5,8,11,14,17- ciseicosapentaenoic acid EPA) production of a bacterium isolated from Pacific mackerel intestines were investigated at 25°C in culture media containing 1.0% peptone and 0.50% yeast extract, and the composition of an optimum culture medium was determined. This bacterium could grow in the media in which sodium chloride was the sole added inorganic component. By raising the concentration of sodium chloride from 1.2 to 2.4%, the yield of bacterial cells increased and the yield of EPA reached a maximum at 2.0% NaCl concentration. In contrast to calcium chloride, potassium chloride and magnesium chloride as second metal salts promoted the growth of this bacterium at relatively low concentrations without inhibiting the accumulation of EPA. The yield of EPA reached its maximum value of 51.9 mg/L of culture broth at 8 hr at 2.0% NaCl, 0.15% KCl and 0.16% MgCl2 concentrations. This yield of EPA was 20% greater than that obtained with Jamarin S artificial sea water.

Original languageEnglish
Pages (from-to)504-508
Number of pages5
JournalJournal of the American Oil Chemists' Society
Volume68
Issue number7
DOIs
Publication statusPublished - 1991 Jul
Externally publishedYes

Fingerprint

Perciformes
Eicosapentaenoic Acid
mackerel
eicosapentaenoic acid
sodium chloride
Intestines
Bacteria
Magnesium Chloride
intestines
Salts
Metals
metals
Sodium chloride
salts
Sodium Chloride
Culture Media
Acids
bacteria
Peptones
Calcium Chloride

Keywords

  • Alteromonas putrefaciens
  • Bacterium
  • Eicosapentaenoic acid
  • Fatty acids
  • Lipids
  • Mackerel

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Food Science
  • Organic Chemistry
  • Chemical Engineering(all)

Cite this

Metal salts requisite for the production of eicosapentaenoic acid by a marine bacterium isolated from mackerel intestines. / Akimoto, Masamichi; Ishii, Tetsuya; Yamagaki, Koji; Ohtaguchi, Kazuhisa; Koide, Kozo; Yazawa, Kazunaga.

In: Journal of the American Oil Chemists' Society, Vol. 68, No. 7, 07.1991, p. 504-508.

Research output: Contribution to journalArticle

Akimoto, Masamichi ; Ishii, Tetsuya ; Yamagaki, Koji ; Ohtaguchi, Kazuhisa ; Koide, Kozo ; Yazawa, Kazunaga. / Metal salts requisite for the production of eicosapentaenoic acid by a marine bacterium isolated from mackerel intestines. In: Journal of the American Oil Chemists' Society. 1991 ; Vol. 68, No. 7. pp. 504-508.
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