Methane production from organic acid-rich wastewaters by immobilized thermophilic methane-producing bacteria

Tadashi Matsunaga, Isao Karube, Shuichi Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)


Thermophilic methane-producing bacteria isolated from a wastewater treatment facility have been immobilized in acetylcellulose filter with agar. The immobilized cells produced methane from wastewaters in rich organic acid (acetic, propionic and butyric acids) at the rate of 1.4 μmol mg protein-1 h-1. The optimum conditions for methane production by immobilized whole cells were 52-55°C and pH 7.0-8.0. The immobilized cells retained 80% of the initial activity after exposure to air. The immobilized thermophilic bacteria produced methane continuously over 10 days at 52°C.

Original languageEnglish
Pages (from-to)441-444
Number of pages4
JournalEnzyme and Microbial Technology
Issue number6
Publication statusPublished - 1983
Externally publishedYes



  • immobilized bacteria
  • Methane production
  • thermophilic bacteria
  • wastewaters

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology

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