Abstract
A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3% (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.
| Original language | English |
|---|---|
| Pages (from-to) | 847-857 |
| Number of pages | 11 |
| Journal | Biotechnology and Bioengineering |
| Volume | 22 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Methane production from wastewaters by immobilized methanogenic bacteria. / Karube, Isao; Kuriyama, Shinichi; Matsunaga, Tadashi; Suzuki, Shuichi.
In: Biotechnology and Bioengineering, Vol. 22, No. 4, 1980, p. 847-857.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Methane production from wastewaters by immobilized methanogenic bacteria
AU - Karube, Isao
AU - Kuriyama, Shinichi
AU - Matsunaga, Tadashi
AU - Suzuki, Shuichi
PY - 1980
Y1 - 1980
N2 - A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3% (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.
AB - A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3% (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.
UR - http://www.scopus.com/inward/record.url?scp=0019008527&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019008527&partnerID=8YFLogxK
U2 - 10.1002/bit.260220409
DO - 10.1002/bit.260220409
M3 - Article
AN - SCOPUS:0019008527
VL - 22
SP - 847
EP - 857
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 4
ER -