Methane production from wastewaters by immobilized methanogenic bacteria

Isao Karube, Shinichi Kuriyama, Tadashi Matsunaga, Shuichi Suzuki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3% (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.

Original languageEnglish
Pages (from-to)847-857
Number of pages11
JournalBiotechnology and Bioengineering
Volume22
Issue number4
DOIs
Publication statusPublished - 1980
Externally publishedYes

Fingerprint

Methanogens
Methane
Waste Water
Wastewater
Bacteria
Gels
Immobilized Cells
Agar
Productivity
Carbohydrates
Polyacrylates
Collagen
Microorganisms
Population
Glucose
Membranes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Methane production from wastewaters by immobilized methanogenic bacteria. / Karube, Isao; Kuriyama, Shinichi; Matsunaga, Tadashi; Suzuki, Shuichi.

In: Biotechnology and Bioengineering, Vol. 22, No. 4, 1980, p. 847-857.

Research output: Contribution to journalArticle

Karube, Isao ; Kuriyama, Shinichi ; Matsunaga, Tadashi ; Suzuki, Shuichi. / Methane production from wastewaters by immobilized methanogenic bacteria. In: Biotechnology and Bioengineering. 1980 ; Vol. 22, No. 4. pp. 847-857.
@article{10d5ef920e554082b0b860c94b656518,
title = "Methane production from wastewaters by immobilized methanogenic bacteria",
abstract = "A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3{\%} (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.",
author = "Isao Karube and Shinichi Kuriyama and Tadashi Matsunaga and Shuichi Suzuki",
year = "1980",
doi = "10.1002/bit.260220409",
language = "English",
volume = "22",
pages = "847--857",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "4",

}

TY - JOUR

T1 - Methane production from wastewaters by immobilized methanogenic bacteria

AU - Karube, Isao

AU - Kuriyama, Shinichi

AU - Matsunaga, Tadashi

AU - Suzuki, Shuichi

PY - 1980

Y1 - 1980

N2 - A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3% (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.

AB - A methanogenic population was immobilized onto agar gel, polyacrylamide gel, and collagen membrane. Agar‐gel‐entrapped methanogenic microorganisms gave the highest activity. The optimum agar concentration was between 1.5 and 3% (w/v), and the optimum microbial content was 20 mg wet cells/g gel. The optimum conditions for methane production by immobilized whole cells were pH 7.0–7.5 and 37–45°C. The rate of methane production was initially 1.8 μmol/g gel/hr. Methane productivity was gradually increased and reached a steady state (4.5μmol/g gel/hr) after 25 days of incubation. The immobilized methanogenic microbial population continuously evolved methane over a 90 day period. No difference in methane productivity was observed after three months of storage at 5°C. Methane was also produced by immobilized whole cells under aerobic conditions. Furthermore, carbohydrates, such as glucose, in wastewater completely decomposed by immobilized whole cells.

UR - http://www.scopus.com/inward/record.url?scp=0019008527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019008527&partnerID=8YFLogxK

U2 - 10.1002/bit.260220409

DO - 10.1002/bit.260220409

M3 - Article

VL - 22

SP - 847

EP - 857

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 4

ER -