Methodological improvements of pyrosequencing technology

Baback Gharizadeh, Michael Akhras, Nader Nourizad, Mehran Ghaderi, Kenji Yasuda, Pål Nyrén, Nader Pourmand

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide polymorphism analysis and sequencing of short stretches of DNA. Although the pyrosequencing procedure is relatively straightforward, users may face challenges due to varying parameters in PCR and sequencing primer design, sample preparation and nucleotide dispensation; such challenges are labor and cost intensive. In this study, these issues have been addressed to increase signal quality and assure sequence accuracy.

Original languageEnglish
Pages (from-to)504-511
Number of pages8
JournalJournal of Biotechnology
Volume124
Issue number3
DOIs
Publication statusPublished - 2006 Jul 25
Externally publishedYes

Fingerprint

DNA
Nucleotides
Technology
DNA Sequence Analysis
Protein Sorting Signals
Polymorphism
Single Nucleotide Polymorphism
Personnel
Costs and Cost Analysis
Polymerase Chain Reaction
Costs

Keywords

  • DNA sequencing
  • Primer-dimers
  • Pyrosequencing technology
  • Sample preparation
  • Single-strand binding protein

ASJC Scopus subject areas

  • Biotechnology

Cite this

Gharizadeh, B., Akhras, M., Nourizad, N., Ghaderi, M., Yasuda, K., Nyrén, P., & Pourmand, N. (2006). Methodological improvements of pyrosequencing technology. Journal of Biotechnology, 124(3), 504-511. https://doi.org/10.1016/j.jbiotec.2006.01.025

Methodological improvements of pyrosequencing technology. / Gharizadeh, Baback; Akhras, Michael; Nourizad, Nader; Ghaderi, Mehran; Yasuda, Kenji; Nyrén, Pål; Pourmand, Nader.

In: Journal of Biotechnology, Vol. 124, No. 3, 25.07.2006, p. 504-511.

Research output: Contribution to journalArticle

Gharizadeh, B, Akhras, M, Nourizad, N, Ghaderi, M, Yasuda, K, Nyrén, P & Pourmand, N 2006, 'Methodological improvements of pyrosequencing technology', Journal of Biotechnology, vol. 124, no. 3, pp. 504-511. https://doi.org/10.1016/j.jbiotec.2006.01.025
Gharizadeh B, Akhras M, Nourizad N, Ghaderi M, Yasuda K, Nyrén P et al. Methodological improvements of pyrosequencing technology. Journal of Biotechnology. 2006 Jul 25;124(3):504-511. https://doi.org/10.1016/j.jbiotec.2006.01.025
Gharizadeh, Baback ; Akhras, Michael ; Nourizad, Nader ; Ghaderi, Mehran ; Yasuda, Kenji ; Nyrén, Pål ; Pourmand, Nader. / Methodological improvements of pyrosequencing technology. In: Journal of Biotechnology. 2006 ; Vol. 124, No. 3. pp. 504-511.
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