Methylation in embryonic stem cells in vitro.

Koichiro Nishino, Jun Ohgane, Masako Suzuki, Naka Hattori, Kunio Shiota

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Stem cells raise the possibility of regenerating failing body parts with new tissue. Before stem cells can safely fulfill their promise, many technical problems, including understanding the stem cell phenotype, must be overcome. DNA methylation, which is responsible for gene silencing and is associated with chromatin remodeling, is an epigenetic system that determines the specific characteristic of a variety of cells, including stem cells. Each cell type has a unique DNA methylation profile produced by varied loci-specific methylation. Investigation of such DNA methylation profiles provides a way of identifying pluripotent stem cells. Further, it is likely that analysis of the epigenetic status of stem cells may provide novel information regarding "sternness" within these populations.

Original languageEnglish
Pages (from-to)421-445
Number of pages25
JournalMethods in molecular biology (Clifton, N.J.)
Volume329
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Embryonic Stem Cells
Methylation
Stem Cells
DNA Methylation
Epigenomics
Pluripotent Stem Cells
Chromatin Assembly and Disassembly
Gene Silencing
Human Body
In Vitro Techniques
Phenotype
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nishino, K., Ohgane, J., Suzuki, M., Hattori, N., & Shiota, K. (2006). Methylation in embryonic stem cells in vitro. Methods in molecular biology (Clifton, N.J.), 329, 421-445.

Methylation in embryonic stem cells in vitro. / Nishino, Koichiro; Ohgane, Jun; Suzuki, Masako; Hattori, Naka; Shiota, Kunio.

In: Methods in molecular biology (Clifton, N.J.), Vol. 329, 2006, p. 421-445.

Research output: Contribution to journalArticle

Nishino, K, Ohgane, J, Suzuki, M, Hattori, N & Shiota, K 2006, 'Methylation in embryonic stem cells in vitro.', Methods in molecular biology (Clifton, N.J.), vol. 329, pp. 421-445.
Nishino K, Ohgane J, Suzuki M, Hattori N, Shiota K. Methylation in embryonic stem cells in vitro. Methods in molecular biology (Clifton, N.J.). 2006;329:421-445.
Nishino, Koichiro ; Ohgane, Jun ; Suzuki, Masako ; Hattori, Naka ; Shiota, Kunio. / Methylation in embryonic stem cells in vitro. In: Methods in molecular biology (Clifton, N.J.). 2006 ; Vol. 329. pp. 421-445.
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