Methylocaldum marinum sp. nov., A thermotolerant, Methane-oxidizing bacterium isolated from marine sediments, And emended description of the genus methylocaldum

Mio Takeuchi, Yoichi Kamagata, Kenshiro Oshima, Satoshi Hanada, Hideyuki Tamaki, Katsumi Marumo, Hiroto Maeda, Munetomo Nedachi, Masahira Hattori, Wataru Iwasaki, Susumu Sakata

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

An aerobic, methane-oxidizing bacterium (strain S8T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is closely related to members of the genus Methylocaldum (97.6-97.9% similarity) within the class Gammaproteobacteria. Strain S8T was a Gram-staining-negative, non-motile, coccoid or short rod-shaped organism. The temperature range for growth of strain S8T was 20-47 °C (optimum growth at 36 °C). It required NaCl (>0.5 %), tolerated up to 5% NaCl and utilized methane and methanol. The major cellular fatty acid and major respiratory quinone were C16 : 0 and 18-methylene ubiquinone 8, respectively. The DNA G+C content was 59.7 mol%. Strain S8T possessed mmoX, which encodes soluble methane monooxygenase, as well as pmoA, which encodes the particulate methane monooxygenase. On the basis of this morphological, physiological, biochemical and genetic information, the first marine species in the genus Methylocaldum is proposed, with the name Methylocaldum marinum sp. nov. The type strain is S8T (5NBRC 109686T5DSM 27392T). An emended description of the genus Methylocaldum is also provided.

Original languageEnglish
Pages (from-to)3240-3246
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
DOIs
Publication statusPublished - 2014 Sep 1
Externally publishedYes

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methane monooxygenase
aprepitant
Methylocaldum
Methylococcaceae
Geologic Sediments
methanotrophs
marine sediments
marine sediment
methane
Gammaproteobacteria
Negative Staining
bacterium
new species
Methane
Base Composition
Growth
rRNA Genes
Names
Methanol
Molecular Biology

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methylocaldum marinum sp. nov., A thermotolerant, Methane-oxidizing bacterium isolated from marine sediments, And emended description of the genus methylocaldum. / Takeuchi, Mio; Kamagata, Yoichi; Oshima, Kenshiro; Hanada, Satoshi; Tamaki, Hideyuki; Marumo, Katsumi; Maeda, Hiroto; Nedachi, Munetomo; Hattori, Masahira; Iwasaki, Wataru; Sakata, Susumu.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, 01.09.2014, p. 3240-3246.

Research output: Contribution to journalArticle

Takeuchi, Mio ; Kamagata, Yoichi ; Oshima, Kenshiro ; Hanada, Satoshi ; Tamaki, Hideyuki ; Marumo, Katsumi ; Maeda, Hiroto ; Nedachi, Munetomo ; Hattori, Masahira ; Iwasaki, Wataru ; Sakata, Susumu. / Methylocaldum marinum sp. nov., A thermotolerant, Methane-oxidizing bacterium isolated from marine sediments, And emended description of the genus methylocaldum. In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64. pp. 3240-3246.
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AU - Kamagata, Yoichi

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AU - Tamaki, Hideyuki

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AU - Maeda, Hiroto

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