Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent

Mio Takeuchi, Taiki Katayama, Takao Yamagishi, Satoshi Hanada, Hideyuki Tamaki, Yoichi Kamagata, Kenshiro Oshima, Masahira Hattori, Katsumi Marumo, Munetomo Nedachi, Hiroto Maeda, Yuichi Suwa, Susumu Sakata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A moderately thermophilic, methanol-oxidizing bacterium (strain Gela4T) was isolated from methane-utilizing mixed-culture originating from marine sediment near a hydrothermal vent. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain Gela4T was closely related to members of the genus 'Methyloligella' (94.7 % similarity) within the class Alphaproteobacteria. Strain Gela4T was a Gram-staining-negative and aerobic organism. Cells were rod-shaped and non-motile. The temperature range for growth of strain Gela4T was 19-43 °C (optimal growth at 35 °C). Strain Gela4T tolerated up to 9 % NaCl with an optimum at 1 %. The organism was a facultative methylotroph that could utilize methanol, methylamine, trimethylamine and a variety of multi-carbon compounds. The major cellular fatty acid and major respiratory quinone were C18: 1ω7c and ubiquinone-10, respectively. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 63.9 mol%. On the basis of the morphological, physiological, biochemical and genetic information, a novel genus and species, Methyloceanibacter caenitepidi is proposed, with Gela4T (= NBRC 109540T = DSM 27242T) as the type strain.

Original languageEnglish
Pages (from-to)462-468
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 2
DOIs
Publication statusPublished - 2014
Externally publishedYes

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Hydrothermal Vents
Geologic Sediments
hydrothermal vent
marine sediments
marine sediment
Methanol
methanol
Bacteria
Alphaproteobacteria
Negative Staining
Phosphatidylglycerols
bacterium
new species
bacteria
Methane
Base Composition
Growth
phospholipid
Phosphatidylcholines
rRNA Genes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. / Takeuchi, Mio; Katayama, Taiki; Yamagishi, Takao; Hanada, Satoshi; Tamaki, Hideyuki; Kamagata, Yoichi; Oshima, Kenshiro; Hattori, Masahira; Marumo, Katsumi; Nedachi, Munetomo; Maeda, Hiroto; Suwa, Yuichi; Sakata, Susumu.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 2, 2014, p. 462-468.

Research output: Contribution to journalArticle

Takeuchi, M, Katayama, T, Yamagishi, T, Hanada, S, Tamaki, H, Kamagata, Y, Oshima, K, Hattori, M, Marumo, K, Nedachi, M, Maeda, H, Suwa, Y & Sakata, S 2014, 'Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent', International Journal of Systematic and Evolutionary Microbiology, vol. 64, no. PART 2, pp. 462-468. https://doi.org/10.1099/ijs.0.053397-0
Takeuchi, Mio ; Katayama, Taiki ; Yamagishi, Takao ; Hanada, Satoshi ; Tamaki, Hideyuki ; Kamagata, Yoichi ; Oshima, Kenshiro ; Hattori, Masahira ; Marumo, Katsumi ; Nedachi, Munetomo ; Maeda, Hiroto ; Suwa, Yuichi ; Sakata, Susumu. / Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64, No. PART 2. pp. 462-468.
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