Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent

Mio Takeuchi, Taiki Katayama, Takao Yamagishi, Satoshi Hanada, Hideyuki Tamaki, Yoichi Kamagata, Kenshiro Oshima, Masahira Hattori, Katsumi Marumo, Munetomo Nedachi, Hiroto Maeda, Yuichi Suwa, Susumu Sakata

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12 Citations (Scopus)

Abstract

A moderately thermophilic, methanol-oxidizing bacterium (strain Gela4T) was isolated from methane-utilizing mixed-culture originating from marine sediment near a hydrothermal vent. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain Gela4T was closely related to members of the genus 'Methyloligella' (94.7 % similarity) within the class Alphaproteobacteria. Strain Gela4T was a Gram-staining-negative and aerobic organism. Cells were rod-shaped and non-motile. The temperature range for growth of strain Gela4T was 19-43 °C (optimal growth at 35 °C). Strain Gela4T tolerated up to 9 % NaCl with an optimum at 1 %. The organism was a facultative methylotroph that could utilize methanol, methylamine, trimethylamine and a variety of multi-carbon compounds. The major cellular fatty acid and major respiratory quinone were C18: 1ω7c and ubiquinone-10, respectively. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 63.9 mol%. On the basis of the morphological, physiological, biochemical and genetic information, a novel genus and species, Methyloceanibacter caenitepidi is proposed, with Gela4T (= NBRC 109540T = DSM 27242T) as the type strain.

Original languageEnglish
Pages (from-to)462-468
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 2
DOIs
Publication statusPublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Takeuchi, M., Katayama, T., Yamagishi, T., Hanada, S., Tamaki, H., Kamagata, Y., Oshima, K., Hattori, M., Marumo, K., Nedachi, M., Maeda, H., Suwa, Y., & Sakata, S. (2014). Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. International Journal of Systematic and Evolutionary Microbiology, 64(PART 2), 462-468. https://doi.org/10.1099/ijs.0.053397-0