Mg2+ dependence of 70 S ribosomal protein flexibility revealed by hydrogen/deuterium exchange and mass spectrometry

Tatsuya Yamamoto, Yoshihiro Shimizu, Takuya Ueda, Yoshitsugu Shiro

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ribosome from Escherichia coli requires a specific concentration of Mg2+ to maintain the 70 S complex formation and allow protein synthesis, and then the structure must be stable and flexible. How does the ribosome acquire these conflicting factors at the same time? Here, we investigated the hydrogen/deuterium exchange of 52 proteins in the 70 S ribosome, which controlled stability and flexibility under various Mg 2+ concentrations, using mass spectrometry. Many proteins exhibited a sigmoidal curve for Mg2+ concentration dependence, incorporating more deuterium at lower Mg2+ concentration. By comparing deuterium incorporation with assembly, we have discovered a typical mechanism of complexes for acquiring both stability and flexibility at the same time. In addition, we got information of the localization of flexibility in ribosomal function by the analysis of related proteins with stalk protein, tRNA, mRNA, and nascent peptide, and demonstrate the relationship between structure, assembly, flexibility, and function of the ribosome.

Original languageEnglish
Pages (from-to)5646-5652
Number of pages7
JournalJournal of Biological Chemistry
Volume285
Issue number8
DOIs
Publication statusPublished - 2010 Feb 19
Externally publishedYes

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Ribosomal Proteins
Deuterium
Mass spectrometry
Hydrogen
Mass Spectrometry
Ribosomes
Proteins
Transfer RNA
Escherichia coli
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mg2+ dependence of 70 S ribosomal protein flexibility revealed by hydrogen/deuterium exchange and mass spectrometry. / Yamamoto, Tatsuya; Shimizu, Yoshihiro; Ueda, Takuya; Shiro, Yoshitsugu.

In: Journal of Biological Chemistry, Vol. 285, No. 8, 19.02.2010, p. 5646-5652.

Research output: Contribution to journalArticle

Yamamoto, Tatsuya ; Shimizu, Yoshihiro ; Ueda, Takuya ; Shiro, Yoshitsugu. / Mg2+ dependence of 70 S ribosomal protein flexibility revealed by hydrogen/deuterium exchange and mass spectrometry. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 8. pp. 5646-5652.
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