Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Masaki Kanai; Hirohsia Abe; Tatsuya Munaka; Y. Yoichi Fujiyama; Daisuke Uchida; Asako Yamayoshi; Hiroaki Nakanishi; Akira Murakami; Shuichi Shoji

doi:10.1016/j.sna.2003.12.006

Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Masaki Kanai^*, Hirohsia Abe, Tatsuya Munaka, Y. Yoichi Fujiyama, Daisuke Uchida, Asako Yamayoshi, Hiroaki Nakanishi, Akira Murakami, Shuichi Shoji

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Contribution to journal › Conference article › peer-review

20 Citations (Scopus)

Abstract

A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

Original language	English
Pages (from-to)	129-134
Number of pages	6
Journal	Sensors and Actuators, A: Physical
Volume	114
Issue number	2-3
DOIs	https://doi.org/10.1016/j.sna.2003.12.006
Publication status	Published - 2004 Sept 1
Event	Selected Papers from Transducers 03 - Boston, MA, United States Duration: 2003 Jun 8 → 2003 Jun 12

Keywords

Cell analysis
Cell culture
Microvalve
Sample injector

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering

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10.1016/j.sna.2003.12.006

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title = "Micro chamber for cellar analysis integrated with negligible dead volume sample injector",

abstract = "A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.",

keywords = "Cell analysis, Cell culture, Microvalve, Sample injector",

author = "Masaki Kanai and Hirohsia Abe and Tatsuya Munaka and Fujiyama, {Y. Yoichi} and Daisuke Uchida and Asako Yamayoshi and Hiroaki Nakanishi and Akira Murakami and Shuichi Shoji",

note = "Funding Information: This work is supported by Research and Development Program for New Bio-Industry Initiatives, of Bio-oriented Technology Research Advancement Institution. This work is partly supported by Japan Ministry of Education, Culture, Sports Science & Technology Grant-in-Aid for COE Research and 21st COE of Waseda University, Scientific Research Priority Area (B) No. 13124209 and Japan Society for the Promotion Science Grant-in-Aid for Creative Scientific Research No. 13GS0024. The authors thank for Nanotechnology Support Project of Waseda University for their technical advices. ; Selected Papers from Transducers 03 ; Conference date: 08-06-2003 Through 12-06-2003",

year = "2004",

month = sep,

day = "1",

doi = "10.1016/j.sna.2003.12.006",

language = "English",

volume = "114",

pages = "129--134",

journal = "Sensors and Actuators A: Physical",

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TY - JOUR

T1 - Micro chamber for cellar analysis integrated with negligible dead volume sample injector

AU - Kanai, Masaki

AU - Abe, Hirohsia

AU - Munaka, Tatsuya

AU - Fujiyama, Y. Yoichi

AU - Uchida, Daisuke

AU - Yamayoshi, Asako

AU - Nakanishi, Hiroaki

AU - Murakami, Akira

AU - Shoji, Shuichi

N1 - Funding Information: This work is supported by Research and Development Program for New Bio-Industry Initiatives, of Bio-oriented Technology Research Advancement Institution. This work is partly supported by Japan Ministry of Education, Culture, Sports Science & Technology Grant-in-Aid for COE Research and 21st COE of Waseda University, Scientific Research Priority Area (B) No. 13124209 and Japan Society for the Promotion Science Grant-in-Aid for Creative Scientific Research No. 13GS0024. The authors thank for Nanotechnology Support Project of Waseda University for their technical advices.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

AB - A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

KW - Cell analysis

KW - Cell culture

KW - Microvalve

KW - Sample injector

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U2 - 10.1016/j.sna.2003.12.006

DO - 10.1016/j.sna.2003.12.006

M3 - Conference article

AN - SCOPUS:4344585455

SN - 0924-4247

VL - 114

SP - 129

EP - 134

JO - Sensors and Actuators A: Physical

JF - Sensors and Actuators A: Physical

IS - 2-3

T2 - Selected Papers from Transducers 03

Y2 - 8 June 2003 through 12 June 2003

ER -

Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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