Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Masaki Kanai, Hirohsia Abe, Tatsuya Munaka, Y. Yoichi Fujiyama, Daisuke Uchida, Asako Yamayoshi, Hiroaki Nakanishi, Akira Murakami, Shuichi Shoji

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

    Original languageEnglish
    Pages (from-to)129-134
    Number of pages6
    JournalSensors and Actuators, A: Physical
    Volume114
    Issue number2-3
    DOIs
    Publication statusPublished - 2004 Sep 1

    Fingerprint

    Reactive ion etching
    injectors
    Etching
    chambers
    Cell culture
    etching
    Rats
    Cells
    rats
    Monitoring
    injection

    Keywords

    • Cell analysis
    • Cell culture
    • Microvalve
    • Sample injector

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering
    • Mechanical Engineering
    • Instrumentation

    Cite this

    Micro chamber for cellar analysis integrated with negligible dead volume sample injector. / Kanai, Masaki; Abe, Hirohsia; Munaka, Tatsuya; Fujiyama, Y. Yoichi; Uchida, Daisuke; Yamayoshi, Asako; Nakanishi, Hiroaki; Murakami, Akira; Shoji, Shuichi.

    In: Sensors and Actuators, A: Physical, Vol. 114, No. 2-3, 01.09.2004, p. 129-134.

    Research output: Contribution to journalArticle

    Kanai, M, Abe, H, Munaka, T, Fujiyama, YY, Uchida, D, Yamayoshi, A, Nakanishi, H, Murakami, A & Shoji, S 2004, 'Micro chamber for cellar analysis integrated with negligible dead volume sample injector', Sensors and Actuators, A: Physical, vol. 114, no. 2-3, pp. 129-134. https://doi.org/10.1016/j.sna.2003.12.006
    Kanai, Masaki ; Abe, Hirohsia ; Munaka, Tatsuya ; Fujiyama, Y. Yoichi ; Uchida, Daisuke ; Yamayoshi, Asako ; Nakanishi, Hiroaki ; Murakami, Akira ; Shoji, Shuichi. / Micro chamber for cellar analysis integrated with negligible dead volume sample injector. In: Sensors and Actuators, A: Physical. 2004 ; Vol. 114, No. 2-3. pp. 129-134.
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    AU - Uchida, Daisuke

    AU - Yamayoshi, Asako

    AU - Nakanishi, Hiroaki

    AU - Murakami, Akira

    AU - Shoji, Shuichi

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