Micro-scale droplet contact method by mechanical motion: Reproducible and robust lipid bilayer formation

L. N S Zaleha, R. Kawano, Hiroki Yasuga, K. Kamiya, T. Osaki, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We prepare a micro-scale droplet contact method by mechanical motion which applying the Split-and-Contact Device (SCD) theory. The interval distance of well opening is designed for limiting the surface area of interface of water droplets. When the droplets are split and joined together, the lipid monolayer surrounding them at the interval combine to form a robust lipid bilayer. The α-hemolysin that acts as pores, incorporate into the lipid bilayer and allows the ionic currents to be flow across lipid bilayer and measured. We present three different interval distance of well opening.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1433-1435
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Lipid bilayers
Contacts (fluid mechanics)
Hemolysin Proteins
Lipids
Monolayers
Water

Keywords

  • Bilayer lipid membrane (BLM)
  • Micro-scale droplet
  • Split-and-Contact Device (SCD)

ASJC Scopus subject areas

  • Bioengineering

Cite this

Zaleha, L. N. S., Kawano, R., Yasuga, H., Kamiya, K., Osaki, T., Miki, N., & Takeuchi, S. (2013). Micro-scale droplet contact method by mechanical motion: Reproducible and robust lipid bilayer formation. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1433-1435). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.

Micro-scale droplet contact method by mechanical motion : Reproducible and robust lipid bilayer formation. / Zaleha, L. N S; Kawano, R.; Yasuga, Hiroki; Kamiya, K.; Osaki, T.; Miki, N.; Takeuchi, S.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1433-1435 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zaleha, LNS, Kawano, R, Yasuga, H, Kamiya, K, Osaki, T, Miki, N & Takeuchi, S 2013, Micro-scale droplet contact method by mechanical motion: Reproducible and robust lipid bilayer formation. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 3, Chemical and Biological Microsystems Society, pp. 1433-1435, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Zaleha LNS, Kawano R, Yasuga H, Kamiya K, Osaki T, Miki N et al. Micro-scale droplet contact method by mechanical motion: Reproducible and robust lipid bilayer formation. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1433-1435. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Zaleha, L. N S ; Kawano, R. ; Yasuga, Hiroki ; Kamiya, K. ; Osaki, T. ; Miki, N. ; Takeuchi, S. / Micro-scale droplet contact method by mechanical motion : Reproducible and robust lipid bilayer formation. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1433-1435 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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